IL-4 and IL-13 Induce Chemotaxis of Human Foreskin Fibroblasts, but Not Human Fetal Lung Fibroblasts

Tadashi Kohyama, Xiangde Liu, Fu Qiang Wen, Tetsu Kobayashi, Shinji Abe, Stephen I. Rennard

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Through shared receptors, IL-4 and IL-13 have been suggested to regulate not only inflammatory cells, but also to play a role in stimulating fibroblasts during fibrotic processes. Previous studies have shown that IL-4 is a chemoattractant for foreskin fibroblasts. The current study was designed to determine the effect of IL-4 and IL-13 on the migration of two types of fibroblasts: foreskin and human fetal lung fibroblasts (HFL-1). Using the Boyden blindwell chamber method, human foreskin or fetal lung fibroblasts (both 106/mL) were placed in upper wells with various concentrations of IL-4 or IL-13 in the lower wells as chemoattractants. Both IL-4 (1 pg/mL) and IL-13 (100 pg/mL) induced foreskin fibroblast chemotaxis, up to 50 ± 8 and 24 ± 7 cells/5 high-power fields, respectively (both p < 0.05). In contrast, neither cytokine induced migration of the lung fibroblasts although both type of cells express IL-4 receptor and IL-13 α1 receptor. These results suggest that fibroblasts are heterogeneous with regard to their ability to respond to cytokine-driven chemotaxis. Therefore, the role of specific cytokines in mediating fibrotic responses might vary depending on local mesenchymal cell responses.

Original languageEnglish (US)
Pages (from-to)33-37
Number of pages5
JournalInflammation
Volume28
Issue number1
DOIs
StatePublished - Feb 1 2004

Keywords

  • Airway remodeling
  • Chemotaxis
  • Fibroblasts
  • IL-13
  • Interleukin-4 (IL-4)

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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