Imaging of DNA and protein–DNA complexes with atomic force microscopy

Yuri L. Lyubchenko, Luda S. Shlyakhtenko

Research output: Contribution to journalReview articlepeer-review

35 Scopus citations


This article reviews atomic force microscopy (AFM) studies of DNA structure and dynamics and protein–DNA complexes, including recent advances in the visualization of protein–DNA complexes with the use of cutting-edge, high-speed AFM. Special emphasis is given to direct nanoscale visualization of dynamics of protein–DNA complexes. In the area of DNA structure and dynamics, structural studies of local non-B conformations of DNA and the interplay of local and global DNA conformations are reviewed. The application of time-lapse AFM nanoscale imaging of DNA dynamics is illustrated by studies of Holliday junction branch migration. Structure and dynamics of protein–DNA interactions include problems related to site-specific DNA recombination, DNA replication, and DNA mismatch repair. Studies involving the structure and dynamics of chromatin are also described.

Original languageEnglish (US)
Pages (from-to)63-96
Number of pages34
JournalCritical Reviews in Eukaryotic Gene Expression
Issue number1
StatePublished - 2016


  • AFM of DNA
  • Atomic force microscopy
  • Chromatin
  • DNA repair
  • DNA replication
  • Holliday junctions
  • Nucleosomes dynamics
  • Protein–DNA complexes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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