Immortalization with telomerase of the Nestin-positive cells of the human pancreas

K. M. Lee, C. Nguyen, A. B. Ulrich, P. M. Pour, M. M. Ouellette

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Cells expressing the neuronal stem cell marker Nestin are present in the human pancreas but the biological role of these cells has yet to be resolved. We report here the establishment with the catalytic subunit of human telomerase (hTERT) of a line of normal human cells representing this cell type. Primary human cells derived from the ducts of the pancreas were transduced with an hTERT cDNA. The infected cells became positive for telomerase, failed to senesce, and were still proliferating after more than 150 doublings. The immortalized cells were positive for the expression of Nestin (at both the mRNA and protein levels) and were found to be free of cancer-associated changes: diploid and expressing wild type p16INK4a, p53, and K-Ras. An established line of normal human cells representing this cell type should be of great value to help define the biological properties of this novel cell type.

Original languageEnglish (US)
Pages (from-to)1038-1044
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume301
Issue number4
DOIs
StatePublished - Feb 21 2003

Keywords

  • Immortalization
  • Islets of Langerhans
  • K-Ras
  • Nestin
  • Pancreas
  • Senescence
  • Telomerase
  • hTERT
  • p16
  • p53

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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