TY - JOUR
T1 - Immune response of pigs inoculated with Mycobacterium bovis BCG expressing a truncated form of GP5 and M protein of porcine reproductive and respiratory syndrome virus
AU - Bastos, Reginaldo G.
AU - Dellagostin, Odir A.
AU - Barletta, Raúl G.
AU - Doster, Allan R.
AU - Nelson, Eric
AU - Zuckermann, Federico
AU - Osorio, Fernando A.
N1 - Funding Information:
R.G. Bastos was supported by Fundação Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior (CAPES Foundation—Brazilian Government). Support for this research project was provided by a grant from the National Research Initiative Competitive Grant Program, project no. 02-35204-12459, of the US department of Agriculture. The publication no. of this paper is 14,064 of the Agricultural Research Division of the Institute of Agriculture and Natural Resources of the University of Nebraska, Lincoln.
PY - 2004/1/2
Y1 - 2004/1/2
N2 - Pigs were immunised with recombinant BCG (rBCG) expressing a truncated form of GP5 (lacking the first 30 NH2-terminal residues) (rBCGGP5) and M protein (rBCGM) of porcine reproductive and respiratory syndrome virus (PRRSV). At 30 days post-inoculation (dpi), pigs inoculated with rBCGGP5 and rBCGM developed a specific humoral immune response against the viral proteins, as detected by commercial ELISA and Western blot tests, and at 60 dpi, three out of five animals developed neutralizing antibodies with titers ranging from 1:4 to 1:8. At 67 dpi, an IFN-γ response against BCG antigens, but not against the viral proteins, was detected by ELISPOT in inoculated pigs. Following challenge with a pathogenic strain of PRRSV, pigs inoculated with rBCG showed lower (P<0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection.
AB - Pigs were immunised with recombinant BCG (rBCG) expressing a truncated form of GP5 (lacking the first 30 NH2-terminal residues) (rBCGGP5) and M protein (rBCGM) of porcine reproductive and respiratory syndrome virus (PRRSV). At 30 days post-inoculation (dpi), pigs inoculated with rBCGGP5 and rBCGM developed a specific humoral immune response against the viral proteins, as detected by commercial ELISA and Western blot tests, and at 60 dpi, three out of five animals developed neutralizing antibodies with titers ranging from 1:4 to 1:8. At 67 dpi, an IFN-γ response against BCG antigens, but not against the viral proteins, was detected by ELISPOT in inoculated pigs. Following challenge with a pathogenic strain of PRRSV, pigs inoculated with rBCG showed lower (P<0.05) temperature, viremia and virus load in bronchial lymph nodes than control animals, suggesting the establishment of partial protection against PRRSV infection.
KW - GP5 and M protein
KW - PRRSV protection
KW - rBCG
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U2 - 10.1016/S0264-410X(03)00572-3
DO - 10.1016/S0264-410X(03)00572-3
M3 - Article
C2 - 14670329
AN - SCOPUS:0345096574
SN - 0264-410X
VL - 22
SP - 467
EP - 474
JO - Vaccine
JF - Vaccine
IS - 3-4
ER -