Immunity-associated programmed cell death as a tool for the identification of genes essential for plant innate immunity

Bangjun Zhou, Lirong Zeng

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Plants have evolved a sophisticated innate immune system to contend with potential infection by various pathogens. Understanding and manipulation of key molecular mechanisms that plants use to defend against various pathogens are critical for developing novel strategies in plant disease control. In plants, resistance to attempted pathogen infection is often associated with hypersensitive response (HR), a form of rapid programmed cell death (PCD) at the site of attempted pathogen invasion. In this chapter, we describe a method for rapid identification of genes that are essential for plant innate immunity. It combines virus-induced gene silencing (VIGS), a tool that is suitable for studying gene function in high-throughput, with the utilization of immunity-associated PCD, particularly HR-linked PCD as the readout of changes in plant innate immunity. The chapter covers from the design of gene fragment for VIGS, the agroinfiltration of the Nicotiana benthamian plants, to the use of immunity-associated PCD induced by twelve elicitors as the indicator of activation of plant immunity.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages51-63
Number of pages13
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1743
ISSN (Print)1064-3745

Keywords

  • Agro-mediated transient expression
  • Nicotiana benthamiana
  • Plant innate immunity
  • Programmed cell death
  • Ubiquitination
  • Virus-induced gene silencing

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Zhou, B., & Zeng, L. (2018). Immunity-associated programmed cell death as a tool for the identification of genes essential for plant innate immunity. In Methods in Molecular Biology (pp. 51-63). (Methods in Molecular Biology; Vol. 1743). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7668-3_5