TY - CHAP
T1 - Immunity-associated programmed cell death as a tool for the identification of genes essential for plant innate immunity
AU - Zhou, Bangjun
AU - Zeng, Lirong
N1 - Funding Information:
This research was supported, in part, by grants from the United States Department of Agriculture (2012-67014-19449) and National Science Foundation (IOS-1645659) to L.Z.
Publisher Copyright:
© 2018, Springer Science+Business Media, LLC.
PY - 2018
Y1 - 2018
N2 - Plants have evolved a sophisticated innate immune system to contend with potential infection by various pathogens. Understanding and manipulation of key molecular mechanisms that plants use to defend against various pathogens are critical for developing novel strategies in plant disease control. In plants, resistance to attempted pathogen infection is often associated with hypersensitive response (HR), a form of rapid programmed cell death (PCD) at the site of attempted pathogen invasion. In this chapter, we describe a method for rapid identification of genes that are essential for plant innate immunity. It combines virus-induced gene silencing (VIGS), a tool that is suitable for studying gene function in high-throughput, with the utilization of immunity-associated PCD, particularly HR-linked PCD as the readout of changes in plant innate immunity. The chapter covers from the design of gene fragment for VIGS, the agroinfiltration of the Nicotiana benthamian plants, to the use of immunity-associated PCD induced by twelve elicitors as the indicator of activation of plant immunity.
AB - Plants have evolved a sophisticated innate immune system to contend with potential infection by various pathogens. Understanding and manipulation of key molecular mechanisms that plants use to defend against various pathogens are critical for developing novel strategies in plant disease control. In plants, resistance to attempted pathogen infection is often associated with hypersensitive response (HR), a form of rapid programmed cell death (PCD) at the site of attempted pathogen invasion. In this chapter, we describe a method for rapid identification of genes that are essential for plant innate immunity. It combines virus-induced gene silencing (VIGS), a tool that is suitable for studying gene function in high-throughput, with the utilization of immunity-associated PCD, particularly HR-linked PCD as the readout of changes in plant innate immunity. The chapter covers from the design of gene fragment for VIGS, the agroinfiltration of the Nicotiana benthamian plants, to the use of immunity-associated PCD induced by twelve elicitors as the indicator of activation of plant immunity.
KW - Agro-mediated transient expression
KW - Nicotiana benthamiana
KW - Plant innate immunity
KW - Programmed cell death
KW - Ubiquitination
KW - Virus-induced gene silencing
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U2 - 10.1007/978-1-4939-7668-3_5
DO - 10.1007/978-1-4939-7668-3_5
M3 - Chapter
C2 - 29332285
AN - SCOPUS:85040862945
T3 - Methods in Molecular Biology
SP - 51
EP - 63
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -