Immunity of the Saccharomyces cerevisiae SSY5 mRNA to nonsense-mediated mRNA decay

Jesseeca Obenoskey, Dakota R. Lane, Audrey L. Atkin, Bessie W. Kebaara

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


The nonsense-mediated mRNA decay (NMD) pathway is a specialized pathway that triggers the rapid degradation of select mRNAs. Initially, identified as a pathway that degrades mRNAs with premature termination codons, NMD is now recognized as a pathway that also regulates some natural mRNAs. Since natural mRNAs do not typically contain premature termination codons, these mRNAs contain features that target them to NMD. In Saccharomyces cerevisiae mRNAs with atypically long 3'-UTRs are usually degraded by NMD, however in some conditions a constitutively expressed SSY5 mRNA with multiple NMD targeting signals including an atypically long 3'-UTR is an exception. We investigated the features of the SSY5 mRNAs that confer immunity to NMD. We found that the SSY5 mRNA 3'-UTRs are sufficient to target NMD insensitive mRNA to the pathway. Replacing the SSY5 3'-UTRs with the cyc1-512 3'-UTRs, known to target mRNAs to NMD or with the CYC1 3'-UTR, known not to target mRNAs to NMD, resulted in production of SSY5 mRNAs that were regulated by NMD. These observations suggest that the SSY5 mRNAs require sequences both within the 5'-UTR and/or ORF as well as the 3'-UTR to escape decay by NMD.

Original languageEnglish (US)
Article number25
JournalFrontiers in Molecular Biosciences
Issue numberDEC
StatePublished - Dec 8 2014


  • 3'-untranslated regions
  • MRNA decay
  • MRNA stability
  • Nonsense-mediated mRNA decay
  • SSY5

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)


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