Abstract
Immunoaffinity chromatography is a process in which the binding affinity of an antigen to a parent antibody is utilized as a basis of separation. Owing to the customized avidity and specificity, monoclonal antibodies (Mabs) have become indispensable for both protein characterization and purification. The immunosorbent performance is dependent on the support matrix upon which the antibody is immobilized and on the activation chemistry used couple the antibody to the matrix. This report details, protocols to immobilize Mabs on commercially available supports, and a method to compute immunosorbent efficiency.
Original language | English (US) |
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Pages (from-to) | 41-47 |
Number of pages | 7 |
Journal | Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology |
Volume | 20 |
Issue number | 1 |
DOIs | |
State | Published - 2002 |
Externally published | Yes |
Keywords
- Antigen
- Antigen-binding efficiency
- Coupling efficiency
- Immunosorbent
- Monoclonal antibodies
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology