Immunoaffinity chromatography: An introduction to applications and recent developments

Annette C. Moser; David S. Hage

doi:10.4155/bio.10.31

Immunoaffinity chromatography: An introduction to applications and recent developments

Annette C. Moser, David S. Hage

Research output: Contribution to journal › Review article

90 Scopus citations

Abstract

Immunoaffinity chromatography (IAC) combines the use of LC with the specific binding of antibodies or related agents. The resulting method can be used in assays for a particular target or for purification and concentration of analytes prior to further examination by another technique. This review discusses the history and principles of IAC and the various formats that can be used with this method. An overview is given of the general properties of antibodies and of antibody-production methods. The supports and immobilization methods used with antibodies in IAC and the selection of application and elution conditions for IAC are also discussed. Several applications of IAC are considered, including its use in purification, immunodepletion, direct sample analysis, chromatographic immunoassays and combined analysis methods. Recent developments include the use of IAC with CE or MS, ultrafast immunoextraction methods and the use of immunoaffinity columns in microanalytical systems.

Original language	English (US)
Pages (from-to)	769-790
Number of pages	22
Journal	Bioanalysis
Volume	2
Issue number	4
DOIs	https://doi.org/10.4155/bio.10.31
State	Published - Apr 2010

ASJC Scopus subject areas

Analytical Chemistry
Pharmacology, Toxicology and Pharmaceutics(all)
Clinical Biochemistry
Medical Laboratory Technology

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Moser, A. C., & Hage, D. S. (2010). Immunoaffinity chromatography: An introduction to applications and recent developments. Bioanalysis, 2(4), 769-790. https://doi.org/10.4155/bio.10.31

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