Immunochemical proof that a novel rearranging gene encodes the T Cell receptor δ subunit

Hamid Band, Frans Hochstenbach, Joanne Mclean, Shingo Hata, Michael S. Krangel, Michael B. Brenner

Research output: Contribution to journalArticle

191 Scopus citations

Abstract

The T cell receptor (TCR) 8 protein is expressed as part of a heterodimer with TCR -y, in association with the CDS potypeptidcs on a subset of functional peripheral blood T lymphocytes, thymocytcs, and certain leukemic T cell lines. A monoclonal antibody directed against TCR 8 was produced that binds specifically to the surface of several TCR 78 cell lines and inununoprecipitates the TCR -yS as a heterodimer from Triton X-100 detergent lysates and also immunoprecipitates the TCR S subunit alone after chain separation. A candidate human TCR 8 complementary DNA done (IDP2 O240/38), reported in a companion paper, was isolated by the subtractive library approach from a TCR γδ cell line. This complementary DNA clone was used to direct the synthesis of a polypeptide that is specifically recognized by the monoclonal antibody to TCR δ. This complementary DNA clone thus corresponds to the gene that encodes the TCR δ subunit.

Original languageEnglish (US)
Pages (from-to)682-684
Number of pages3
JournalScience
Volume238
Issue number4827
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

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    Band, H., Hochstenbach, F., Mclean, J., Hata, S., Krangel, M. S., & Brenner, M. B. (1987). Immunochemical proof that a novel rearranging gene encodes the T Cell receptor δ subunit. Science, 238(4827), 682-684. https://doi.org/10.1126/science.3672118