Immunochemical studies of conjugates of isomaltosyl oligosaccharides to lipid: Fractionation of rabbit antibodies to stearyl-isomaltosyl oligosaccharides and a study of their combining sites by a competitive binding assay

Charles Wood, Elvin A. Kabat

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

An antiserum to stearyl-IM5 liposomes, R-856, with a high proportion of IgM and IgG antibodies was separated into IgM and IgG fractions by column chromatography. The IgM and IgG antibody binding sites were studied by quantitative precipitin, precipitin inhibition and competitive binding assays. Both were similar to unfractionated antibodies with sites complementary to IM4. Another antiserum, R-846, was separated into antibody fractions of restricted heterogeneity by preparative isoelectric focusing and their combining sites were studied by competitive binding. Two groups of site sizes were found, one complementary to IM5 was similar to the unfractionated antiserum whereas the other had smaller size sites complementary to IM4.

Original languageEnglish (US)
Pages (from-to)277-289
Number of pages13
JournalArchives of Biochemistry and Biophysics
Volume212
Issue number1
DOIs
StatePublished - Nov 1981

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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