An antiserum to stearyl-IM5 liposomes, R-856, with a high proportion of IgM and IgG antibodies was separated into IgM and IgG fractions by column chromatography. The IgM and IgG antibody binding sites were studied by quantitative precipitin, precipitin inhibition and competitive binding assays. Both were similar to unfractionated antibodies with sites complementary to IM4. Another antiserum, R-846, was separated into antibody fractions of restricted heterogeneity by preparative isoelectric focusing and their combining sites were studied by competitive binding. Two groups of site sizes were found, one complementary to IM5 was similar to the unfractionated antiserum whereas the other had smaller size sites complementary to IM4.
ASJC Scopus subject areas
- Molecular Biology