TY - JOUR
T1 - Immunohistochemical expression of mucin antigens in gallbladder adenocarcinoma
T2 - MUC1-positive and MUC2-negative expression is associated with vessel invasion and shortened survival
AU - Hiraki, Tsubasa
AU - Yamada, Sohsuke
AU - Higashi, Michiyo
AU - Hatanaka, Kazuhito
AU - Yokoyama, Seiya
AU - Kitazono, Ikumi
AU - Goto, Yuko
AU - Kirishima, Mari
AU - Batra, Surinder K.
AU - Yonezawa, Suguru
AU - Tanimoto, Akihide
N1 - Funding Information:
Acknowledgemts. We would like to thank Dr. Yoshikazu Harada, DDS, Department of Pathology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan; and Department of Dentistry and Oral Surgery, University Hospital of Occupational and Environmental Health, Kitakyushu 807-8555, Japan, for his excellent and various technical/statistical assistance and helpful comments, and Kei Matsuo, Orie Iwaya and Yoshie Jitoho for their expert technical assistance, respectively. Financial support. This work was supported in part by Fukuoka Foundation for Sound Health Cancer Research Fund, Fukuoka, Japan and a grant from the Kodama Memorial Fund for Medical Research, Kagoshima, Japan (to S.Yamada), and Grants-in-Aid for Scientific Research on Scientific Research (C) 15K08466 (to M.H.) and Young Scientists (B) 15K21247 to (S.Yokoyama) from the Ministry of Education, Science, Sports, Culture and Technology, Japan. Conflict of interest. No competing financial interests exist.
PY - 2017/6
Y1 - 2017/6
N2 - Mucins play pivotal roles in influencing cancer biology, for example affecting carcinoma invasion, aggressiveness and/or metastatic potential. Our aim is to investigate the significance of expression profiles of two mucins in particular, MUC1 and MUC2, their correlations with various clinicopathological features, and prognosis in gallbladder adenocarcinoma (GBAC). We performed immunohistochemistry from patients with surgically resected GBAC, using antibodies against mucin core proteins MUC1/DF3 and MUC2/Ccp58 in 81 paraffin-embedded tumor samples. MUC1 or MUC2 expression was considered to be high when ≥20% or 10% of the GBAC cells showed positive staining, respectively. High MUC1 expression was revealed to have a significant relationship to the presence of pathologically lymphatic and vascular invasion, and regional lymph node metastasis. By contrast, high MUC2 expression showed a significant correlation with pathologically perineural invasion, T stage ≥3, and post-operative recurrence. Moreover, MUC1 showed significantly positive co-expression and potentially complementary correlations with MUC2. Multivariate analyses demonstrated that the high MUC1 expression group had significantly shorter disease-specific survival times. However, the combination of both high MUC1 and MUC2 expression did not predict worse outcome in GBACs. Therefore, although each mucin has a somewhat important role in the pathogenesis of GBAC progression, MUC1 can independently predict vessel invasion and poor prognosis in patients with GBAC. The detection of MUC1 might well offer a useful parameter for providing clinical management and treatment against postsurgical GBACs.
AB - Mucins play pivotal roles in influencing cancer biology, for example affecting carcinoma invasion, aggressiveness and/or metastatic potential. Our aim is to investigate the significance of expression profiles of two mucins in particular, MUC1 and MUC2, their correlations with various clinicopathological features, and prognosis in gallbladder adenocarcinoma (GBAC). We performed immunohistochemistry from patients with surgically resected GBAC, using antibodies against mucin core proteins MUC1/DF3 and MUC2/Ccp58 in 81 paraffin-embedded tumor samples. MUC1 or MUC2 expression was considered to be high when ≥20% or 10% of the GBAC cells showed positive staining, respectively. High MUC1 expression was revealed to have a significant relationship to the presence of pathologically lymphatic and vascular invasion, and regional lymph node metastasis. By contrast, high MUC2 expression showed a significant correlation with pathologically perineural invasion, T stage ≥3, and post-operative recurrence. Moreover, MUC1 showed significantly positive co-expression and potentially complementary correlations with MUC2. Multivariate analyses demonstrated that the high MUC1 expression group had significantly shorter disease-specific survival times. However, the combination of both high MUC1 and MUC2 expression did not predict worse outcome in GBACs. Therefore, although each mucin has a somewhat important role in the pathogenesis of GBAC progression, MUC1 can independently predict vessel invasion and poor prognosis in patients with GBAC. The detection of MUC1 might well offer a useful parameter for providing clinical management and treatment against postsurgical GBACs.
KW - Disease-specific survival
KW - Gallbladder adenocarcinoma (GBAC)
KW - MUC1
KW - MUC2
KW - Vessel invasion
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U2 - 10.14670/HH-11-824
DO - 10.14670/HH-11-824
M3 - Article
C2 - 27672051
AN - SCOPUS:85015200367
VL - 32
SP - 585
EP - 596
JO - Histology and Histopathology
JF - Histology and Histopathology
SN - 0213-3911
IS - 6
ER -