Immunohistochemistry of pancreatic neoplasia

Sukhwinder Kaur, Tomohiro Shimizu, Michael J. Baine, Sushil Kumar, Surinder K. Batra

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Immunohistochemistry (IHC) is a valuable tool to visualize the distribution and localization of specificcellular components within morphologically preserved tissue sections or cell preparations. It combines the histologic morphology of tissues for detecting the actual antigen distribution, specificity of antibody-antigen interaction for optimal detection, and sensitivity of immunochemical methods for assessing the amount of antigen in tissues. It is routinely used clinically to diagnose type (benign or malignant), stage, and grade of cancer using specific tumor markers. The application of IHC ranges from disease diagnosis and prognosis to drug development and analysis of the pathobiological roles of various molecular players during disease development. Due to better availability of highly specific antibodies and optimal methodologies for performing immunohistochemical studies, IHC is being used at an expanding rate to understand pancreatic tumor biology as well as to study the fate of various molecular markers during the initiation, progression, and metastasis of pancreatic neoplasia. Herein, we describe the detailed protocol for IHC analyses of pancreatic intraepithelial neoplasia in tissues and fine needle aspirates from both human and mouse samples.

Original languageEnglish (US)
Title of host publicationPancreatic Cancer
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages29-42
Number of pages14
ISBN (Print)9781627032865
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume980
ISSN (Print)1064-3745

Keywords

  • Antibodies
  • Fixation
  • Immunohistochemistry
  • Mucins
  • Pancreatic cancer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Kaur, S., Shimizu, T., Baine, M. J., Kumar, S., & Batra, S. K. (2013). Immunohistochemistry of pancreatic neoplasia. In Pancreatic Cancer: Methods and Protocols (pp. 29-42). (Methods in Molecular Biology; Vol. 980). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-287-2_3