Immunologic attributes of cytokine mobilized peripheral blood stem cells and recovery following transplantation

J. E. Talmadge, E. C. Reed, A. Kessinger, C. A. Kuszynski, G. A. Perry, C. L. Gordy, K. C. Mills, M. L. Thomas, S. J. Pirruccello, B. A. Letheby, M. A. Arneson, J. D. Jackson

Research output: Contribution to journalArticlepeer-review

91 Scopus citations


The immunologic attributes of cytokine mobilized peripheral blood stem cell (PSC) products (n = 52) and the resulting reconstitution of the hematopoietic and immunologic system following autologous transplantation were examined in a consecutive population of non-Hodgkin lymphoma (NHL), or solid tumor patients at the University of Nebraska Medical Center. Granulocyte-monocyte colony stimulating factor (GM-CSF)-mobilized PSC products had a high frequency of monocytes (31%) and bands (15%) as compared to normal peripheral blood (PB) cells. The phenotypic analysis of the mobilized PSC product revealed that they had normal levels of CD4+ cells, an increased frequency of CD8+ cells and a corresponding decrease in the CD4+:CD8+ cell ratio as compared to the peripheral blood leukocytes (PBL) of normal individuals. PSC products also had an increase in CD34+ cells as compared to PB. Natural killer (NK) and T cell activity in the PSC products were also lower than that observed in PB. Post-transplantation there was an accelerated reconstitution of NK-cell function in the PB as compared to T cell function (PHA (phytohemagglutinin) mitogenesis) which did not return to normal by day 100 post-transplantation. We also report for the first time high levels of an irradiation resistant suppressor cell activity in the PSC product and in the PB post-transplantation. There was also a concomitant increase in CD4-, CD8-, TCR α/β+ cells (phenotypic homolog of 'natural suppressor' (NS) cells) in the PB post-transplantation. The number of months of prior chemotherapy correlated with PHA response but the NS activity and frequency of CD4-, CD8- and TCR α/β+ cells did not. Further, cytokine mobilization and apheresis appears to contribute to the loss of PHA responsiveness and the increased levels of suppressor cell activity.

Original languageEnglish (US)
Pages (from-to)101-109
Number of pages9
JournalBone marrow transplantation
Issue number1
StatePublished - Jan 1996


  • CD34
  • FACS
  • Immune function
  • Natural suppressor cells
  • Peripheral blood stem cell transplantation

ASJC Scopus subject areas

  • Hematology
  • Transplantation


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