Several methods for quantitating the reaction of the antihistone sera with purified chromatin and isolated nucleosomes have been devised. With these antisera it is possible to visualize the in situ organization of histones in nuclei, metaphase chromosomes and polytene chromosomes by the immunofluorescence technique. The organization of histone at the level of the single nucleosome in repressed, transcribed, and replicating regions of the genome can be resolved by immunoelectron microscopic techniques. In this paper the authors describe their studies on the in situ organization of histones in chromosomes and chromatin. In addition, the authors present evidence that, analogous to histone antibodies, lectins can be used as probes for chromatin structure.
ASJC Scopus subject areas
- Molecular Biology