Impact of beta 2-microglobulin on tapasin expression and covalent association

Laura C. Simone; Brittney L. Smith; Joyce C. Solheim

doi:10.1016/j.cellimm.2012.09.010

Impact of beta 2-microglobulin on tapasin expression and covalent association

Laura C. Simone, Brittney L. Smith, Joyce C. Solheim

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Cellular immunity is dependent on major histocompatibility complex (MHC) class I molecules enabling cytotoxic T cell recognition of malignant and infected cells. Loading of antigenic peptides onto MHC class I is assisted by a peptide-loading protein complex including tapasin. We found that tapasin expression is enhanced by beta 2-microglobulin via both transcriptional and post-transcriptional mechanisms. In addition, using conditions which preserve the tapasin-ERp57 disulfide-bonded conjugate, we demonstrated that beta 2-microglobulin increases tapasin-containing protein complexes, and reduces the level of MHC class I/ERp57 complexes lacking tapasin. Overall, our results provide a new perspective on the regulation of tapasin expression and association.

Original language	English (US)
Pages (from-to)	66-69
Number of pages	4
Journal	Cellular Immunology
Volume	279
Issue number	1
DOIs	https://doi.org/10.1016/j.cellimm.2012.09.010
State	Published - Sep 2012

Keywords

Antigen presentation
Beta 2-microglobulin
D
ERp57
Major histocompatibility complex class I
Peptide-loading complex
Tapasin

ASJC Scopus subject areas

Immunology

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10.1016/j.cellimm.2012.09.010

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title = "Impact of beta 2-microglobulin on tapasin expression and covalent association",

abstract = "Cellular immunity is dependent on major histocompatibility complex (MHC) class I molecules enabling cytotoxic T cell recognition of malignant and infected cells. Loading of antigenic peptides onto MHC class I is assisted by a peptide-loading protein complex including tapasin. We found that tapasin expression is enhanced by beta 2-microglobulin via both transcriptional and post-transcriptional mechanisms. In addition, using conditions which preserve the tapasin-ERp57 disulfide-bonded conjugate, we demonstrated that beta 2-microglobulin increases tapasin-containing protein complexes, and reduces the level of MHC class I/ERp57 complexes lacking tapasin. Overall, our results provide a new perspective on the regulation of tapasin expression and association.",

keywords = "Antigen presentation, Beta 2-microglobulin, D, ERp57, Major histocompatibility complex class I, Peptide-loading complex, Tapasin",

author = "Simone, {Laura C.} and Smith, {Brittney L.} and Solheim, {Joyce C.}",

note = "Funding Information: This work was supported by NIH Grants R01 GM057428 and GM057428-09S1, a Nebraska DHHS grant, and the Nebraska Center for Cellular Signaling Grants from the National Center for Research Resources (P20 RR18759) and the National Institute of General Medical Sciences (P20 GM103489) (J.C.S.), and a UNMC Graduate Studies/Presidential Fellowship and an NIH/NCI Training Grant T32 CA009476 Fellowship (to L.C.S.). The funding sources had no role in the study design, in the collection, analysis, and interpretation of the data, in the writing of the report, or in the decision to submit the article for publication. We gratefully acknowledge the assistance of Peter Simone with the statistical analysis and Briana Ormsbee with the qRT-PCR assays.",

year = "2012",

month = sep,

doi = "10.1016/j.cellimm.2012.09.010",

language = "English (US)",

volume = "279",

pages = "66--69",

journal = "Cellular Immunology",

issn = "0008-8749",

publisher = "Academic Press Inc.",

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AU - Simone, Laura C.

AU - Smith, Brittney L.

AU - Solheim, Joyce C.

N1 - Funding Information: This work was supported by NIH Grants R01 GM057428 and GM057428-09S1, a Nebraska DHHS grant, and the Nebraska Center for Cellular Signaling Grants from the National Center for Research Resources (P20 RR18759) and the National Institute of General Medical Sciences (P20 GM103489) (J.C.S.), and a UNMC Graduate Studies/Presidential Fellowship and an NIH/NCI Training Grant T32 CA009476 Fellowship (to L.C.S.). The funding sources had no role in the study design, in the collection, analysis, and interpretation of the data, in the writing of the report, or in the decision to submit the article for publication. We gratefully acknowledge the assistance of Peter Simone with the statistical analysis and Briana Ormsbee with the qRT-PCR assays.

PY - 2012/9

Y1 - 2012/9

N2 - Cellular immunity is dependent on major histocompatibility complex (MHC) class I molecules enabling cytotoxic T cell recognition of malignant and infected cells. Loading of antigenic peptides onto MHC class I is assisted by a peptide-loading protein complex including tapasin. We found that tapasin expression is enhanced by beta 2-microglobulin via both transcriptional and post-transcriptional mechanisms. In addition, using conditions which preserve the tapasin-ERp57 disulfide-bonded conjugate, we demonstrated that beta 2-microglobulin increases tapasin-containing protein complexes, and reduces the level of MHC class I/ERp57 complexes lacking tapasin. Overall, our results provide a new perspective on the regulation of tapasin expression and association.

AB - Cellular immunity is dependent on major histocompatibility complex (MHC) class I molecules enabling cytotoxic T cell recognition of malignant and infected cells. Loading of antigenic peptides onto MHC class I is assisted by a peptide-loading protein complex including tapasin. We found that tapasin expression is enhanced by beta 2-microglobulin via both transcriptional and post-transcriptional mechanisms. In addition, using conditions which preserve the tapasin-ERp57 disulfide-bonded conjugate, we demonstrated that beta 2-microglobulin increases tapasin-containing protein complexes, and reduces the level of MHC class I/ERp57 complexes lacking tapasin. Overall, our results provide a new perspective on the regulation of tapasin expression and association.

KW - Antigen presentation

KW - Beta 2-microglobulin

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KW - Major histocompatibility complex class I

KW - Peptide-loading complex

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Impact of beta 2-microglobulin on tapasin expression and covalent association

Abstract

Keywords

ASJC Scopus subject areas

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