Impact of beta 2-microglobulin on tapasin expression and covalent association

Laura C. Simone, Brittney L. Smith, Joyce C. Solheim

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Cellular immunity is dependent on major histocompatibility complex (MHC) class I molecules enabling cytotoxic T cell recognition of malignant and infected cells. Loading of antigenic peptides onto MHC class I is assisted by a peptide-loading protein complex including tapasin. We found that tapasin expression is enhanced by beta 2-microglobulin via both transcriptional and post-transcriptional mechanisms. In addition, using conditions which preserve the tapasin-ERp57 disulfide-bonded conjugate, we demonstrated that beta 2-microglobulin increases tapasin-containing protein complexes, and reduces the level of MHC class I/ERp57 complexes lacking tapasin. Overall, our results provide a new perspective on the regulation of tapasin expression and association.

Original languageEnglish (US)
Pages (from-to)66-69
Number of pages4
JournalCellular Immunology
Volume279
Issue number1
DOIs
StatePublished - Sep 2012

Keywords

  • Antigen presentation
  • Beta 2-microglobulin
  • D
  • ERp57
  • Major histocompatibility complex class I
  • Peptide-loading complex
  • Tapasin

ASJC Scopus subject areas

  • Immunology

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