Impaired T and NK cell response of bone marrow and peripheral blood stem cell products to interleukin (IL)-2

Ibrahim Ozerol, Ana Ageitos, Dean G. Heimann, James E. Talmadge

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The function of steady-state and interleukin (IL)-2-co-cultured mononuclear cells differs significantly between bone marrow (BM) products, growth factor-mobilized peripheral blood stem cell (PSC) products and normal peripheral blood mononuclear cells (PBMC). The natural killer (NK) cell activity and T cell proliferative response of PSC products from non-Hodgkin's lymphoma (NHL) patients are significantly higher than that of BM products and similar to normal PBMC. However, following a five-day co-culture with IL-2 (100 IU/ml), the NK activity of PSC, PBMC, and BM products (lytic units) was increased 176-, 40-, and 14-fold, respectively, compared to that observed prior to IL-2 culture. In contrast, lymphokine activated killer (LAK) cytotoxicity prior to IL-2 culture was low in PSC and BM products and normal PBMC, but was significantly increased in PSC products and PBMC following IL-2 co-culture. The proliferative response of PSC and BM products to the T cell mitogen phytohemagglutinin (PHA) was significantly lower than that observed with normal PBMC; however, PSC had a significantly higher response than cells from BM products. Similar patterns of T cell PHA mitogenic response were observed after IL-2 co-culture. In addition, the IL-2 mitogenic responses of IL-2-co-cultured PSC and BM products were also significantly lower than that observed with PBMC co-cultured with IL-2. The IL-2 mitogenic response of PBMC was also significantly increased compared to prior to IL-2 co-culture; whereas, the IL-2 mitogenic responses from PSC and BM cells were not. In summary, co-culture with IL-2 can increase the NK and LAK cell cytotoxicity of PSC and BM products from NHL patients, but IL-2 co-culture does not improve T cell function within either BM or PSC products. Copyright (C) 1999 International Society for Immunopharmacology.

Original languageEnglish (US)
Pages (from-to)509-521
Number of pages13
JournalInternational Journal of Immunopharmacology
Volume21
Issue number8
DOIs
StatePublished - Aug 1 1999

Keywords

  • Bone marrow transplantation
  • IL-2
  • LAK
  • NK
  • Non-Hodgkin's lymphoma
  • PHA
  • Peripheral stem cell transplantation
  • T cell

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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