Impulsive pressurization of neuronal cells for traumatic brain injury study

Matthew Nienaber, Jeong Soon Lee, Ruqiang Feng, Jung Yul Lim

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic brain injury (TBI). We demonstrate in this video article how blast TBI-relevant impulsive pressurization is applied to the neuronal cells in vitro. This is achieved by using well-controlled pressure pulse created by a specialized Kolsky bar device, with complete pressure history within the cell pressurization chamber recorded. Pressurized neuronal cells are inspected immediately after pressurization, or further incubated to examine the long-term effects of impulsive pressurization on neurite/axonal outgrowth, neuronal gene expression, apoptosis, etc. We observed that impulsive pressurization at about 2 MPa induces distinct neurite loss relative to unpressurized cells. Our technique provides a novel method to investigate the molecular/cellular mechanisms of blast TBI, via impulsive pressurization of brain cells at well-controlled pressure magnitude and duration.

Original languageEnglish (US)
Article numbere2723
Pages (from-to)1-4
Number of pages4
JournalJournal of Visualized Experiments
Issue number56
DOIs
StatePublished - Oct 2011

Keywords

  • Bioengineering
  • Blast-tbi
  • Impulsive pressurization
  • Issue 56
  • Neuronal cells
  • Neurons
  • Neuroscience
  • Traumatic brain injury

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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