In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films

Yi Zou; Lei Wan; Jenifer Blacklock; David Oupicky; Guangzhao Mao

doi:10.1007/978-1-62703-140-0-13

In situ AFM analysis investigating disassembly of DNA nanoparticles and nano-films

Yi Zou, Lei Wan, Jenifer Blacklock, David Oupicky, Guangzhao Mao

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Synthetic vector-based gene delivery systems continue to gain strength as viable alternatives to viral vectors due to safety and other concerns. DNA release dynamics is key to the understanding and control of gene delivery from nano-systems. Here we describe atomic force microscope application to the understanding of DNA release dynamics from bioreducible polycation-based nano-systems. The two nano-systems are polyplex nanoparticles and layer-by-layer fi lms.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Pages	183-193
Number of pages	11
Volume	948
DOIs	https://doi.org/10.1007/978-1-62703-140-0-13
State	Published - 2013
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	948
ISSN (Print)	10643745

Keywords

DNA release
Gene delivery
In situ AFM
Layer-by-layer film
Polyplex

ASJC Scopus subject areas

Molecular Biology
Genetics
Medicine(all)

Access to Document

10.1007/978-1-62703-140-0-13

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abstract = "Synthetic vector-based gene delivery systems continue to gain strength as viable alternatives to viral vectors due to safety and other concerns. DNA release dynamics is key to the understanding and control of gene delivery from nano-systems. Here we describe atomic force microscope application to the understanding of DNA release dynamics from bioreducible polycation-based nano-systems. The two nano-systems are polyplex nanoparticles and layer-by-layer fi lms.",

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AU - Oupicky, David

AU - Mao, Guangzhao

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