In situ AFM analysis investigating disassembly of DNA nanoparticles and nanofilms

Yi Zou, Lei Wan, Jenifer Blacklock, Lingxiao Xie, Sean Carroll, David Oupicky, Guangzhao Mao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Synthetic vector-based gene delivery continues to gain strength as viable alternatives to viral vectors due to safety and other concerns. DNA release dynamics is key to the understanding and control of gene delivery from nanosystems. Here we describe atomic force microscope (AFM) application to the understanding of DNA release dynamics from bioreducible polycation-based nanosystems. The two nanosystems are polyplex nanoparticles and layer-by-layer (LbL) films.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages199-209
Number of pages11
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1943
ISSN (Print)1064-3745

Keywords

  • DNA release
  • Gene delivery
  • In situ AFM
  • Layer-by-layer (LbL) film
  • Polyplex

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Zou, Y., Wan, L., Blacklock, J., Xie, L., Carroll, S., Oupicky, D., & Mao, G. (2019). In situ AFM analysis investigating disassembly of DNA nanoparticles and nanofilms. In Methods in Molecular Biology (pp. 199-209). (Methods in Molecular Biology; Vol. 1943). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9092-4_13