In situ hybridization for the detection and localization of swine Chlamydia trachomatis

C. Chae, D. S. Cheon, D. Kwon, O. Kim, B. Kim, J. Suh, D. G. Rogers, K. D.E. Everett, A. A. Andersen

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Gnotobiotic piglets were inoculated intralaryngeally with swine Chlamydia trachomatis strain R33 or orally with swine C. trachmatis strain R27. Archived formalin-fixed, paraffin-embedded tissues from piglets euthanatized 4-7 days postinoculation were examined by in situ hybridization for C. trachomatis nucleic acid using a nonradioactive digoxigenin-labeled DNA probes that targeted specific ribosomal RNA or omp l mRNA molecules of the swine C. trachomatis strains. Positive hybridization signals were detected in bronchial epithelial cells, bronchiolar epithelial cells, pneumocytes, alveolar and interstitial macrophages, and jejunal and ileal enterocytes. Chlamydia-infected cells had a strong signal that was confined to the intracytoplasmic inclusions. Positive hybridization signals were not detected in tissue sections from an uninfected control piglet or in C. psittaci-infected sheep placenta. The morphology of host cells was preserved despite the relatively high temperature required in parts of the incubation procedure. The data indicate that in situ hybridization can be used to detect swine C. trachomatis in formalin-fixed, paraffin-embedded tissue specimens.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalVeterinary pathology
Volume36
Issue number2
DOIs
StatePublished - 1999

Keywords

  • Chlamydia trachomatis
  • Enteritis
  • In situ hybridization
  • Pneumonia
  • Swine

ASJC Scopus subject areas

  • veterinary(all)

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