TY - GEN
T1 - In situ visualization of dynamic interactions of cellulase and cellulose molecules
AU - Song, Bo
AU - Xi, Ning
AU - Yang, Ruiguo
AU - Sun, Zhiyong
AU - Chen, Liangliang
N1 - Publisher Copyright:
© 2014 IEEE.
PY - 2014/11/26
Y1 - 2014/11/26
N2 - Enzymatic hydrolysis of cellulose from biomass is potentially considered as a new approach for the next generation of biofuels for sustainable energy. However, because of the rigid and complex structure of cellulose, the low efficiency of the enzymatic hydrolysis has been deemed as the bottleneck in biorefining industry. The mechanism of enzymatic hydrolysis of cellulose is not well understood due to the absence of direct observation methods. In the present study, in order to study the mechanism of enzymatic hydrolysis of cellulose, we used an atomic force microscopy (AFM) to dynamically observe the changes happening during the cellulase and cellulose interaction. To improve the imaging rate of AFM (which was too slow to capture the interactions between these two nano structures), a scan strategy named compressive scan is used, associated with prior knowledge based image reconstruction methods. With the help of compressive scan, we are able to dynamically capture the action of the cellulase-CBH I (from family 7 cellobiohydrolase) on the cellulose crystal nanofibrils. The result is helpful for understanding the mechanism of enzymatic interaction and increasing the efficiency of hydrolysis process.
AB - Enzymatic hydrolysis of cellulose from biomass is potentially considered as a new approach for the next generation of biofuels for sustainable energy. However, because of the rigid and complex structure of cellulose, the low efficiency of the enzymatic hydrolysis has been deemed as the bottleneck in biorefining industry. The mechanism of enzymatic hydrolysis of cellulose is not well understood due to the absence of direct observation methods. In the present study, in order to study the mechanism of enzymatic hydrolysis of cellulose, we used an atomic force microscopy (AFM) to dynamically observe the changes happening during the cellulase and cellulose interaction. To improve the imaging rate of AFM (which was too slow to capture the interactions between these two nano structures), a scan strategy named compressive scan is used, associated with prior knowledge based image reconstruction methods. With the help of compressive scan, we are able to dynamically capture the action of the cellulase-CBH I (from family 7 cellobiohydrolase) on the cellulose crystal nanofibrils. The result is helpful for understanding the mechanism of enzymatic interaction and increasing the efficiency of hydrolysis process.
KW - Atomic force microscopy
KW - Cellulase
KW - Cellulose
KW - Enzymatic hydrolysis
KW - Fast scan
KW - In situ visualization
UR - http://www.scopus.com/inward/record.url?scp=84919484557&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84919484557&partnerID=8YFLogxK
U2 - 10.1109/NANO.2014.6968134
DO - 10.1109/NANO.2014.6968134
M3 - Conference contribution
AN - SCOPUS:84919484557
T3 - Proceedings of the IEEE Conference on Nanotechnology
SP - 13
EP - 17
BT - Proceedings of the IEEE Conference on Nanotechnology
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2014 14th IEEE International Conference on Nanotechnology, IEEE-NANO 2014
Y2 - 18 August 2014 through 21 August 2014
ER -