In vitro culture of fetal ovaries: A model to study factors regulating early follicular development

Shyamal K. Roy, Cheng Wang, Anindit Mukherjee, Prabuddha Chakraborty

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Follicular development commences with the formation of primordial follicles, which begins with the differentiation of pluripotent ovarian somatic cells into early granulosa cells and their apposition to the oocytes in the egg nest. The process of primordial follicle morphogenesis and factors affecting the formation and development of primordial follicles can be examined in vitro using fetal ovaries in organ culture. The functions of candidate genes involved in primordial folliculogenesis can be examined using siRNA or shRNA, which can knockdown specific mRNA targets at specific time points. Here, we describe the organ culture protocol for fetal hamster ovary with GPR30 siRNA as an example. The method to morphologically analyze follicular development is also discussed.

Original languageEnglish (US)
Title of host publicationGermline Development
Subtitle of host publicationMethods and Protocols
EditorsWai-Yee Chan, Le Ann Blomberg
Pages151-171
Number of pages21
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume825
ISSN (Print)1064-3745

Keywords

  • In vitro
  • Method
  • Oocytes
  • Primordial follicle
  • siRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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