In vitro embryo development of good and poor quality bovine oocytes following microinjection of DNA

A. S. Garst, S. K. Carlin, J. R. Glbbons, K. W. Kendrick, R. E. Pearson, R. M. Akers, T. L. Bailey, W. H. Velander, H. K. Lubon, F. C. Gwazdauskas

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Embryo development of different quality oocytes retrieved by transvaginal follicular aspiration (TVFA) and following microinjection of DNA was examined. Embryos from good quality TVFA-oocytes had higher development scores than embryos from poor quality oocytes, but were not different from good quality slaughter house derived (SH) oocytes. Embryo development following injection of DNA was the same for oocytes in each source-quality group (TVFA-good, 8.4%; TVFA-poor, 5.5%; SH-good, 6.3% blastocysts). Oocytes from TVFA produced more polymerase chain reaction (PCR) positive blastocysts (95.0%) than SH-oocytes (61.5%). More calves were born from the transfer of embryos injected with DNA and resulting from TVFA-oocytes (3/5) than from SH-oocytes (1/6), although not statistically significant. The use of good quality oocytes obtained by TVFA may improve the efficiency of producing transgenic cattle.

Original languageEnglish (US)
Pages (from-to)371-382
Number of pages12
JournalTransgenics
Volume2
Issue number4
StatePublished - 1999

Keywords

  • Bovine
  • DNA
  • Microinjection
  • Oocyte development
  • PCR

ASJC Scopus subject areas

  • Genetics

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