In vitro inhibition of trophoblast maturation and expansion of early rat blastocysts by an oestrogen antagonist

S. K. Roy, J. Sengupta, B. C. Paria, S. K. Manchanda

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Rat blastocyst development and expansion were studied in an in vitro culture system. Early blastocysts collected from rats on the morning of day 5 were shown to develop normally and underwent expansion when placed in bovine serum ablumin supplemented culture medium for 12 h at 37°C. Nafoxidine, and oestrogen antagonist, prevented embryo expansion when added to the culture medium, and it was found that if oestradiol-17β was added to this medium this inhibitory effect was removed and the embryos again expanded. Ethanol, used as a solvent for steroids, and cholesterol were added to the medium containing nafoxidine but the blastocysts still remained unexpanded after 12 h in culture. These results suggest a need for oestrogen action during blastocyst expansion in vitro. Cultured rat blastocysts were subjected to histochemical study for acid phosphatase (ACPase) and leucylnaphthylamidase (LNAse). In unexpanded blastocysts ACPase was found to be low while LNAse activity was higher when compared with enzyme distribution patterns in expanded blastocysts.

Original languageEnglish (US)
Pages (from-to)129-135
Number of pages7
JournalActa Endocrinologica
Volume99
Issue number1
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Endocrinology

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