In vitro myelin formation using embryonic stem cells

Bilal E. Kerman; Hyung Joon Kim; Krishnan Padmanabhan; Arianna Mei; Shereen Georges; Matthew S. Joens; James A.J. Fitzpatrick; Roberto Jappelli; Karen J. Chandross; Paul August; Fred H. Gage

doi:10.1242/dev.116517

In vitro myelin formation using embryonic stem cells

Bilal E. Kerman, Hyung Joon Kim, Krishnan Padmanabhan, Arianna Mei, Shereen Georges, Matthew S. Joens, James A.J. Fitzpatrick, Roberto Jappelli, Karen J. Chandross, Paul August, Fred H. Gage

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Myelination in the central nervous system is the process by which oligodendrocytes form myelin sheaths around the axons of neurons. Myelination enables neurons to transmit information more quickly and more efficiently and allows for more complex brain functions; yet, remarkably, the underlying mechanism by which myelination occurs is still not fully understood. A reliable in vitro assay is essential to dissect oligodendrocyte and myelin biology. Hence, we developed a protocol to generate myelinating oligodendrocytes from mouse embryonic stem cells and established a myelin formation assay with embryonic stem cell-derived neurons in microfluidic devices. Myelin formation was quantified using a custom semi-automated method that is suitable for larger scale analysis. Finally, early myelination was followed in real time over several days and the results have led us to propose a new model for myelin formation.

Original language	English (US)
Pages (from-to)	2213-2225
Number of pages	13
Journal	Development (Cambridge)
Volume	142
Issue number	12
DOIs	https://doi.org/10.1242/dev.116517
State	Published - Jun 15 2015
Externally published	Yes

Keywords

Automated quantification
Embryonic stem cells
Live imaging
Myelin
Oligodendrocyte

ASJC Scopus subject areas

Molecular Biology
Developmental Biology

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10.1242/dev.116517

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title = "In vitro myelin formation using embryonic stem cells",

abstract = "Myelination in the central nervous system is the process by which oligodendrocytes form myelin sheaths around the axons of neurons. Myelination enables neurons to transmit information more quickly and more efficiently and allows for more complex brain functions; yet, remarkably, the underlying mechanism by which myelination occurs is still not fully understood. A reliable in vitro assay is essential to dissect oligodendrocyte and myelin biology. Hence, we developed a protocol to generate myelinating oligodendrocytes from mouse embryonic stem cells and established a myelin formation assay with embryonic stem cell-derived neurons in microfluidic devices. Myelin formation was quantified using a custom semi-automated method that is suitable for larger scale analysis. Finally, early myelination was followed in real time over several days and the results have led us to propose a new model for myelin formation.",

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AU - Kerman, Bilal E.

AU - Kim, Hyung Joon

AU - Padmanabhan, Krishnan

AU - Mei, Arianna

AU - Georges, Shereen

AU - Joens, Matthew S.

AU - Fitzpatrick, James A.J.

AU - Jappelli, Roberto

AU - Chandross, Karen J.

AU - August, Paul

AU - Gage, Fred H.

PY - 2015/6/15

Y1 - 2015/6/15

N2 - Myelination in the central nervous system is the process by which oligodendrocytes form myelin sheaths around the axons of neurons. Myelination enables neurons to transmit information more quickly and more efficiently and allows for more complex brain functions; yet, remarkably, the underlying mechanism by which myelination occurs is still not fully understood. A reliable in vitro assay is essential to dissect oligodendrocyte and myelin biology. Hence, we developed a protocol to generate myelinating oligodendrocytes from mouse embryonic stem cells and established a myelin formation assay with embryonic stem cell-derived neurons in microfluidic devices. Myelin formation was quantified using a custom semi-automated method that is suitable for larger scale analysis. Finally, early myelination was followed in real time over several days and the results have led us to propose a new model for myelin formation.

AB - Myelination in the central nervous system is the process by which oligodendrocytes form myelin sheaths around the axons of neurons. Myelination enables neurons to transmit information more quickly and more efficiently and allows for more complex brain functions; yet, remarkably, the underlying mechanism by which myelination occurs is still not fully understood. A reliable in vitro assay is essential to dissect oligodendrocyte and myelin biology. Hence, we developed a protocol to generate myelinating oligodendrocytes from mouse embryonic stem cells and established a myelin formation assay with embryonic stem cell-derived neurons in microfluidic devices. Myelin formation was quantified using a custom semi-automated method that is suitable for larger scale analysis. Finally, early myelination was followed in real time over several days and the results have led us to propose a new model for myelin formation.

KW - Automated quantification

KW - Embryonic stem cells

KW - Live imaging

KW - Myelin

KW - Oligodendrocyte

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In vitro myelin formation using embryonic stem cells

Abstract

Keywords

ASJC Scopus subject areas

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