In vitro propagation of a Himalayan pine P. wallichiana A. B. Jacks

Four different plant tissue culture media containing varying levels of growth hormones were studied to determine the nutritional and hormonal requirements for induction and subsequent elongation of shoot buds and rooting. Germinating embryos as explants placed in an inverted position in LP medium containing BAP produced adventitious shoot buds. Activated charcoal treatment of the embryos after being exposed to BAP was necessary for shoot proliferation and elongation. Elongated shoot buds were passed to rooting media. While using this technique for micropropagation of other pines, it would be necessary to optimize the age of the explant and the duration of exposure in shoot induction medium.