In vitro translation products of mrnas derived from TMV-infected tobacco exhibiting a hypersensitive response

Thomas E. Smart; David D. Dunigan; Milton Zaitlin

doi:10.1016/0042-6822(87)90222-4

In vitro translation products of mrnas derived from TMV-infected tobacco exhibiting a hypersensitive response

Thomas E. Smart, David D. Dunigan, Milton Zaitlin

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Expression of the hypersensitive response (HSR) to tobacco mosaic virus (TMV) infection in Nicotiana tabacum L. cv. Xanthi-nc (genotype NN) is controlled by the single dominant "N" gene and is temperature sensitive. TMV-infected Xanthi-nc tobacco plants grown at the HSR-restrictive temperature of 31° for 3 days postinoculation show necrosis approximately 8 hr after the temperature shift to the HSR-permissive temperature of 25°. Both polyribosomal and total cytoplasmic poly(A)-containing RNAs were isolated at various times after the temperature shift from leaves of TMV-infected and mock-infected Xanthi-nc tobacco plants and from TMV-infected Turkish Samsun tobacco plants (genotype nn; systemic for TMV infection). The RNAs were translated in vitro and the products were analyzed by polyacrylamide gel electrophoresis. A minimum of four polypeptide translation products specific to both the polyribosomal and total cytoplasmic poly(A)-containing RNAs derived from hypersensitively responding Xanthi-nc were observed.

Original language	English (US)
Pages (from-to)	461-464
Number of pages	4
Journal	Virology
Volume	158
Issue number	2
DOIs	https://doi.org/10.1016/0042-6822(87)90222-4
State	Published - Jun 1987
Externally published	Yes

ASJC Scopus subject areas

Virology

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10.1016/0042-6822(87)90222-4

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@article{fc1d827616c84b399c72d9e1fe134563,

title = "In vitro translation products of mrnas derived from TMV-infected tobacco exhibiting a hypersensitive response",

abstract = "Expression of the hypersensitive response (HSR) to tobacco mosaic virus (TMV) infection in Nicotiana tabacum L. cv. Xanthi-nc (genotype NN) is controlled by the single dominant {"}N{"} gene and is temperature sensitive. TMV-infected Xanthi-nc tobacco plants grown at the HSR-restrictive temperature of 31° for 3 days postinoculation show necrosis approximately 8 hr after the temperature shift to the HSR-permissive temperature of 25°. Both polyribosomal and total cytoplasmic poly(A)-containing RNAs were isolated at various times after the temperature shift from leaves of TMV-infected and mock-infected Xanthi-nc tobacco plants and from TMV-infected Turkish Samsun tobacco plants (genotype nn; systemic for TMV infection). The RNAs were translated in vitro and the products were analyzed by polyacrylamide gel electrophoresis. A minimum of four polypeptide translation products specific to both the polyribosomal and total cytoplasmic poly(A)-containing RNAs derived from hypersensitively responding Xanthi-nc were observed.",

author = "Smart, {Thomas E.} and Dunigan, {David D.} and Milton Zaitlin",

note = "Funding Information: These studies were supported in part by Grant 84-09851 from the National Science Foundation. David Dunigan holds a postdoctoral fellowship from the Cornell Biotechnoloy Program. We thank P. Pa-lukaitis and N. Young for their interest and valuable discussions and T. M. A. Wilson for his consideration of this manuscript. M. Brodie for her help with its preparation.",

year = "1987",

month = jun,

doi = "10.1016/0042-6822(87)90222-4",

language = "English (US)",

volume = "158",

pages = "461--464",

journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - In vitro translation products of mrnas derived from TMV-infected tobacco exhibiting a hypersensitive response

AU - Smart, Thomas E.

AU - Dunigan, David D.

AU - Zaitlin, Milton

N1 - Funding Information: These studies were supported in part by Grant 84-09851 from the National Science Foundation. David Dunigan holds a postdoctoral fellowship from the Cornell Biotechnoloy Program. We thank P. Pa-lukaitis and N. Young for their interest and valuable discussions and T. M. A. Wilson for his consideration of this manuscript. M. Brodie for her help with its preparation.

PY - 1987/6

Y1 - 1987/6

N2 - Expression of the hypersensitive response (HSR) to tobacco mosaic virus (TMV) infection in Nicotiana tabacum L. cv. Xanthi-nc (genotype NN) is controlled by the single dominant "N" gene and is temperature sensitive. TMV-infected Xanthi-nc tobacco plants grown at the HSR-restrictive temperature of 31° for 3 days postinoculation show necrosis approximately 8 hr after the temperature shift to the HSR-permissive temperature of 25°. Both polyribosomal and total cytoplasmic poly(A)-containing RNAs were isolated at various times after the temperature shift from leaves of TMV-infected and mock-infected Xanthi-nc tobacco plants and from TMV-infected Turkish Samsun tobacco plants (genotype nn; systemic for TMV infection). The RNAs were translated in vitro and the products were analyzed by polyacrylamide gel electrophoresis. A minimum of four polypeptide translation products specific to both the polyribosomal and total cytoplasmic poly(A)-containing RNAs derived from hypersensitively responding Xanthi-nc were observed.

AB - Expression of the hypersensitive response (HSR) to tobacco mosaic virus (TMV) infection in Nicotiana tabacum L. cv. Xanthi-nc (genotype NN) is controlled by the single dominant "N" gene and is temperature sensitive. TMV-infected Xanthi-nc tobacco plants grown at the HSR-restrictive temperature of 31° for 3 days postinoculation show necrosis approximately 8 hr after the temperature shift to the HSR-permissive temperature of 25°. Both polyribosomal and total cytoplasmic poly(A)-containing RNAs were isolated at various times after the temperature shift from leaves of TMV-infected and mock-infected Xanthi-nc tobacco plants and from TMV-infected Turkish Samsun tobacco plants (genotype nn; systemic for TMV infection). The RNAs were translated in vitro and the products were analyzed by polyacrylamide gel electrophoresis. A minimum of four polypeptide translation products specific to both the polyribosomal and total cytoplasmic poly(A)-containing RNAs derived from hypersensitively responding Xanthi-nc were observed.

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DO - 10.1016/0042-6822(87)90222-4

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VL - 158

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JO - Virology

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ER -

In vitro translation products of mrnas derived from TMV-infected tobacco exhibiting a hypersensitive response

Abstract

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