In Vivo Binding of Single-Stranded DNA-Binding Protein to Stalled Replication Fork Helicases

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Understanding protein-protein interactions is key to unraveling protein function in vivo. Here we describe a dual/triple-plasmid system that enables co-expression of two, or three, recombinant proteins harboring different affinity tags in the same Escherichia coli cell. This novel protein expression system provides a platform to understand protein-protein interactions and enables researchers to study protein complex formation and in vivo localization.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages81-91
Number of pages11
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2281
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • DNA helicase
  • PriA
  • RecG
  • Single-strand binding protein
  • SSB
  • Stalled fork rescue

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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