Abstract
Detection of nitric oxide (NO) in vivo by single-walled carbon nanotubes (SWNT) is based on the fluorescent properties of SWNT and the ability of NO to quench the fluorescence signal. Alterations of the signal can be utilized to detect a small molecule in vivo that has not previously been possible by other assay techniques. The protocols described here explain the techniques used to prepare NO-detecting SWNTs and to administer them to mice by both intravenous and subcutaneous routes. These techniques can also be utilized with other SWNT sensors as well as non-SWNT sensors.
Original language | English (US) |
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Pages (from-to) | 93-102 |
Number of pages | 10 |
Journal | Current protocols in chemical biology |
Volume | 7 |
Issue number | 2 |
DOIs | |
State | Published - Jun 1 2015 |
Externally published | Yes |
Keywords
- hydrogel
- in vivo
- intravenous
- nitric oxide
- sensor
- single-walled carbon nanotubes
- subcutaneous
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology