@article{a485e4b3dcaa4147922376f09df4a06f,
title = "In vivo effect of wood smoke on the expression of two mucin genes in rat airways",
abstract = "A short-term, time-dependent smoke exposure of rats in a nose-only chamber to burning wood and 24-h recovery time revealed inflammation of the airways with varying degrees of injury from loss of cilia, degeneration of epithelium, and squamous metaplasia to submucosal edema. These histological changes were reflected in variable expression of the secretory Muc5AC and low expression of membrane-associated Muc4 mucin genes. 20-min smoke exposure in extended recovery experiments showed marked disorder of tracheal epithelium for up to 72 h of recovery with a return to normal by 7 days. Gene expressions were elevated at 24 and 48 h of recovery. 30-min smoke exposure showed a more severe degeneration of the epithelium and a longer recovery time. Muc5AC expression decreased after 72 h of recovery, while there was upregulation of Muc4 gene from 48 through 96 h. Because Muc4 upregulation and histological results correlate and it has reportedly been associated with epithelium renewal, Muc4 gene may be a useful marker for the regeneration of tracheal epithelium.",
keywords = "lungs, rats, smoke exposure, trachea",
author = "Bhattacharyya, {Sambhu N.} and Dubick, {Michael A.} and Yantis, {Loudon D.} and Enriquez, {John I.} and Buchanan, {Kelvin C.} and Batra, {Surinder K.} and Smiley, {Rebecca A.}",
note = "Funding Information: Acknowledgments—We thank Drs. Idelle Weisman and Sang Pak of the Department of Clinical Investigation, William Beaumont Army Medical Center for their help and encouragement. We appreciate the assistance of the staffs of the Biological Research Service, Department of Clinical Investigation, William Beaumont Army Medical Center for providing tracheal samples. We thank the staffs of the Visual Information Branch, William Beaumont Army Medical Center and especially Mr. Raul Hernandez for providing us with all the illustrations. We appreciate the technical assistance of Mr. David Cameron of U.S. Army Institute of Surgical Research with biochemical analysis. We thank Dr. Anthony E. Pusateri of U.S. Army Institute of Surgical Research for his interest and advise. This study was funded by a grant from Combat Casualty Care Research Program, USAMRMC (MM4556HB1). The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Department of the army or the Department of Defense",
year = "2004",
month = apr,
doi = "10.1023/B:IFLA.0000033022.66289.04",
language = "English (US)",
volume = "28",
pages = "67--76",
journal = "Inflammation",
issn = "0360-3997",
publisher = "Springer New York",
number = "2",
}