TY - GEN
T1 - In vivo Magnetic Resonance Elastography of mesenchymally derived constructs
AU - Othman, Shadi F.
AU - Curtis, Evan T.
AU - Xu, Huihui
PY - 2011
Y1 - 2011
N2 - The multidisciplinary field of tissue engineering (TE) aims to create biological substitutes to restore, maintain, or enhance the function of tissue or whole organs, in which the mechanical properties of replacements are critical to their structure and function. Several techniques have been investigated for measuring the mechanical properties of developing tissues and organs; however, current methods are destructive. The goal of this work is to apply high resolution Magnetic Resonance Elastography (MRE) technique with 100 m in-plane resolution to address a critical problem in TE: nondestructive monitoring and characterization of growing mesenchymal stem cells (MSCs)-based tissue engineered constructs. In this study, MRE is used to assess the development of osteogenic and adipogenic constructs implanted in immunodeficient nude mice. In vivo MRE experiments were conducted at 9.4 T (400 MHz for protons) using an 89 mm vertical bore magnet. Multiple shear waves were presented in the adipogenic constructs in comparison to the osteogenic constructs. This study demonstrated that MRE can be used to non-invasively monitor the growth and development of various types of engineered tissues in vivo.
AB - The multidisciplinary field of tissue engineering (TE) aims to create biological substitutes to restore, maintain, or enhance the function of tissue or whole organs, in which the mechanical properties of replacements are critical to their structure and function. Several techniques have been investigated for measuring the mechanical properties of developing tissues and organs; however, current methods are destructive. The goal of this work is to apply high resolution Magnetic Resonance Elastography (MRE) technique with 100 m in-plane resolution to address a critical problem in TE: nondestructive monitoring and characterization of growing mesenchymal stem cells (MSCs)-based tissue engineered constructs. In this study, MRE is used to assess the development of osteogenic and adipogenic constructs implanted in immunodeficient nude mice. In vivo MRE experiments were conducted at 9.4 T (400 MHz for protons) using an 89 mm vertical bore magnet. Multiple shear waves were presented in the adipogenic constructs in comparison to the osteogenic constructs. This study demonstrated that MRE can be used to non-invasively monitor the growth and development of various types of engineered tissues in vivo.
KW - Magnetic Resonance Elastography (MRE)
KW - Mechanical property
KW - Stiffness
KW - Tissue Engineering (TE)
UR - http://www.scopus.com/inward/record.url?scp=84863035794&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863035794&partnerID=8YFLogxK
U2 - 10.1109/ITiME.2011.6130914
DO - 10.1109/ITiME.2011.6130914
M3 - Conference contribution
AN - SCOPUS:84863035794
SN - 9781612847023
T3 - ITME 2011 - Proceedings: 2011 IEEE International Symposium on IT in Medicine and Education
SP - 621
EP - 624
BT - ITME 2011 - Proceedings
T2 - 2011 IEEE International Symposium on IT in Medicine and Education, ITME 2011
Y2 - 9 December 2011 through 11 December 2011
ER -