In vivo osteopontin-induced macrophage accumulation is dependent on CD44 expression

Maria Cecilia G. Marcondes, Matthew Poling, Debbie D. Watry, De Shon Hall, Howard S. Fox

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In order to assess the role of osteopontin (OPN) in leukocyte accumulation in inflammatory conditions, native OPN and its thrombin cleaved form (OPN + Thr) were studied in vivo using a rodent subcutaneous air pouch model (AP). Both forms of OPN-induced macrophage infiltration into the AP in wild-type mice. In animals lacking CD44, macrophage numbers were significantly reduced within the cavity, but cells still accumulated along the subcutaneous lining. In animals lacking endogenous OPN, no differences were found in exogenous OPN-induced macrophage accumulation, although macrophage exhibited increased α4 integrin expression. These studies reveal that both OPN and OPN + Thr attract macrophages in vivo through CD44.

Original languageEnglish (US)
Pages (from-to)56-62
Number of pages7
JournalCellular Immunology
Volume254
Issue number1
DOIs
StatePublished - Aug 4 2008

    Fingerprint

Keywords

  • CD44
  • Chemotaxis
  • Macrophages
  • Osteopontin

ASJC Scopus subject areas

  • Immunology

Cite this