TY - JOUR
T1 - Increase in the relative abundance of preproenkephalin A messenger RNA in the ventricles of cardiomyopathic hamsters
AU - Ouellette, Michel
AU - Brakier-Gingras, Léa
N1 - Funding Information:
We wish to thank Drs. Guy Boileau, Luc DesGroseillers and Vicenzo De Luca for helpful discussions and comments. The skilful secretarial assistance of Ms Lorraine Charette is greatly appreciated and we thank Ms Christine Ostiguy for the photographs of the autoradiograms. This work was supported by the Fondation Blanche Cloutier. Michel Ouellette is a recipient of a studentship from the Medical Research Council of Canada.
PY - 1988/8/30
Y1 - 1988/8/30
N2 - Preproenkephalin A messenger RNA was detected in hamster heart by Northern blot analysis using a human preproenkephalin A cDNA probe. Ventricular levels of this messenger were one order of magnitude lower than atrial levels, which were equivalent to brain levels. Furthermore, in the heart of cardiomyopathic hamsters, an animal model of cardiac hypertrophy and congestive heart failure, the relative abundance of the preproenkephalin A messenger RNA was found to increase three- to four-fold in ventricles while no change was seen in atria. These results support the hypothesis that the heart has the potential for locally synthesizing enkephalins and provide evidence that alterations in preproenkephalin A messenger RNA levels are associated with the development of cardiac hypertrophy and failure.
AB - Preproenkephalin A messenger RNA was detected in hamster heart by Northern blot analysis using a human preproenkephalin A cDNA probe. Ventricular levels of this messenger were one order of magnitude lower than atrial levels, which were equivalent to brain levels. Furthermore, in the heart of cardiomyopathic hamsters, an animal model of cardiac hypertrophy and congestive heart failure, the relative abundance of the preproenkephalin A messenger RNA was found to increase three- to four-fold in ventricles while no change was seen in atria. These results support the hypothesis that the heart has the potential for locally synthesizing enkephalins and provide evidence that alterations in preproenkephalin A messenger RNA levels are associated with the development of cardiac hypertrophy and failure.
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U2 - 10.1016/S0006-291X(88)81107-0
DO - 10.1016/S0006-291X(88)81107-0
M3 - Article
C2 - 3415700
AN - SCOPUS:0023814349
SN - 0006-291X
VL - 155
SP - 449
EP - 454
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -