Increase of the LC-MS/MS sensitivity and detection limits using on-line sample preparation with large volume plasma injection

Ming Li, Yazen Alnouti, Rachael Leverence, Honggang Bi, Arkady I. Gusev

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Large volume injection (LVI) has systematically been studied to improve LC-MS/MS sensitivity (signal-to-noise ratio, or S/N) and detection limits. The method of LVI was combined with on-line solid phase extraction (on-line SPE) and LC-MS/MS detection for analysis of compounds directly in plasma. It was demonstrated that LVI of plasma with on-line SPE-LC-MS/MS allows for improvement of sensitivity and detection limits without compromising chromatographic peak shape and resolution and inducing significant matrix and signal suppression effects. Furthermore, sensitivity and detection limits improve linearly with the injection volume up to 100 μL. Quantification of the model compounds in plasma demonstrated comparable calibration curve statistics, precision and accuracy for 5, 50 and 100 μL plasma injections.

Original languageEnglish (US)
Pages (from-to)152-160
Number of pages9
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume825
Issue number2
DOIs
StatePublished - Oct 25 2005

Keywords

  • Bioanalytical
  • LC-MS/MS
  • Large volume injection
  • On-line SPE
  • Sensitivity improvement

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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