Increased rectal microbial richness is associated with the presence of colorectal adenomas in humans

Nina Sanapareddy; Ryan M. Legge; Biljana Jovov; Amber McCoy; Lauren Burcal; Felix Araujo-Perez; Thomas A. Randall; Joseph Galanko; Andrew Benson; Robert S. Sandler; John F. Rawls; Zaid Abdo; Anthony A. Fodor; Temitope O. Keku

doi:10.1038/ismej.2012.43

Increased rectal microbial richness is associated with the presence of colorectal adenomas in humans

Nina Sanapareddy, Ryan M. Legge, Biljana Jovov, Amber McCoy, Lauren Burcal, Felix Araujo-Perez, Thomas A. Randall, Joseph Galanko, Andrew Benson, Robert S. Sandler, John F. Rawls, Zaid Abdo, Anthony A. Fodor, Temitope O. Keku

Food Science and Technology

Research output: Contribution to journal › Article › peer-review

174 Scopus citations

Abstract

Differences in the composition of the gut microbial community have been associated with diseases such as obesity, Crohns disease, ulcerative colitis and colorectal cancer (CRC). We used 454 titanium pyrosequencing of the V1-V2 region of the 16S rRNA gene to characterize adherent bacterial communities in mucosal biopsy samples from 33 subjects with adenomas and 38 subjects without adenomas (controls). Biopsy samples from subjects with adenomas had greater numbers of bacteria from 87 taxa than controls; only 5 taxa were more abundant in control samples. The magnitude of the differences in the distal gut microbiota between patients with adenomas and controls was more pronounced than that of any other clinical parameters including obesity, diet or family history of CRC. This suggests that sequence analysis of the microbiota could be used to identify patients at risk for developing adenomas.

Original language	English (US)
Pages (from-to)	1858-1868
Number of pages	11
Journal	ISME Journal
Volume	6
Issue number	10
DOIs	https://doi.org/10.1038/ismej.2012.43
State	Published - Oct 2012

Keywords

BMI
Colon cancer
Commensal bacteria
Diagnostic

ASJC Scopus subject areas

Microbiology
Ecology, Evolution, Behavior and Systematics

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10.1038/ismej.2012.43

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Sanapareddy, N., Legge, R. M., Jovov, B., McCoy, A., Burcal, L., Araujo-Perez, F., Randall, T. A., Galanko, J., Benson, A., Sandler, R. S., Rawls, J. F., Abdo, Z., Fodor, A. A., & Keku, T. O. (2012). Increased rectal microbial richness is associated with the presence of colorectal adenomas in humans. ISME Journal, 6(10), 1858-1868. https://doi.org/10.1038/ismej.2012.43

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title = "Increased rectal microbial richness is associated with the presence of colorectal adenomas in humans",

abstract = "Differences in the composition of the gut microbial community have been associated with diseases such as obesity, Crohns disease, ulcerative colitis and colorectal cancer (CRC). We used 454 titanium pyrosequencing of the V1-V2 region of the 16S rRNA gene to characterize adherent bacterial communities in mucosal biopsy samples from 33 subjects with adenomas and 38 subjects without adenomas (controls). Biopsy samples from subjects with adenomas had greater numbers of bacteria from 87 taxa than controls; only 5 taxa were more abundant in control samples. The magnitude of the differences in the distal gut microbiota between patients with adenomas and controls was more pronounced than that of any other clinical parameters including obesity, diet or family history of CRC. This suggests that sequence analysis of the microbiota could be used to identify patients at risk for developing adenomas.",

keywords = "BMI, Colon cancer, Commensal bacteria, Diagnostic",

author = "Nina Sanapareddy and Legge, {Ryan M.} and Biljana Jovov and Amber McCoy and Lauren Burcal and Felix Araujo-Perez and Randall, {Thomas A.} and Joseph Galanko and Andrew Benson and Sandler, {Robert S.} and Rawls, {John F.} and Zaid Abdo and Fodor, {Anthony A.} and Keku, {Temitope O.}",

note = "Funding Information: This research was supported, in part, by grants from the National Institutes of Health NIH P30DK034987, R01 CA44684, P50 CA106991, R01 CA136887, K01 DK 073695. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. Nucleotide sequence accession numbers: All 454 pyrose-quence data from this study have been submitted to Genbank database under the accession # SRS 166138. 1-172960.2.",

year = "2012",

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doi = "10.1038/ismej.2012.43",

language = "English (US)",

volume = "6",

pages = "1858--1868",

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AU - Abdo, Zaid

AU - Fodor, Anthony A.

AU - Keku, Temitope O.

N1 - Funding Information: This research was supported, in part, by grants from the National Institutes of Health NIH P30DK034987, R01 CA44684, P50 CA106991, R01 CA136887, K01 DK 073695. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. Nucleotide sequence accession numbers: All 454 pyrose-quence data from this study have been submitted to Genbank database under the accession # SRS 166138. 1-172960.2.

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AB - Differences in the composition of the gut microbial community have been associated with diseases such as obesity, Crohns disease, ulcerative colitis and colorectal cancer (CRC). We used 454 titanium pyrosequencing of the V1-V2 region of the 16S rRNA gene to characterize adherent bacterial communities in mucosal biopsy samples from 33 subjects with adenomas and 38 subjects without adenomas (controls). Biopsy samples from subjects with adenomas had greater numbers of bacteria from 87 taxa than controls; only 5 taxa were more abundant in control samples. The magnitude of the differences in the distal gut microbiota between patients with adenomas and controls was more pronounced than that of any other clinical parameters including obesity, diet or family history of CRC. This suggests that sequence analysis of the microbiota could be used to identify patients at risk for developing adenomas.

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Increased rectal microbial richness is associated with the presence of colorectal adenomas in humans

Abstract

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