Abstract
To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 4008-4011 |
| Number of pages | 4 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 90 |
| Issue number | 9 |
| DOIs | |
| State | Published - May 1 1993 |
Keywords
- Catalysis
- Cofactors
- Coordination complex
- Transgenic mice
- Transition metals
ASJC Scopus subject areas
- General
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Cite this
Sarvetnick, N., Gurushanthaiah, D., Han, N., Prudent, J., Schultz, P., & Lerner, R. (1993). Increasing the chemical potential of the germ-line antibody repertoire. Proceedings of the National Academy of Sciences of the United States of America, 90(9), 4008-4011. https://doi.org/10.1073/pnas.90.9.4008