Increasing the chemical potential of the germ-line antibody repertoire

Nora Sarvetnick, Deepak Gurushanthaiah, Nianhe Han, James Prudent, Peter Schultz, Richard Lerner

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.

Original languageEnglish (US)
Pages (from-to)4008-4011
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number9
DOIs
StatePublished - May 1 1993

Keywords

  • Catalysis
  • Cofactors
  • Coordination complex
  • Transgenic mice
  • Transition metals

ASJC Scopus subject areas

  • General

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