Increasing the chemical potential of the germ-line antibody repertoire

Nora Sarvetnick; Deepak Gurushanthaiah; Nianhe Han; James Prudent; Peter Schultz; Richard Lerner

doi:10.1073/pnas.90.9.4008

Increasing the chemical potential of the germ-line antibody repertoire

Nora Sarvetnick, Deepak Gurushanthaiah, Nianhe Han, James Prudent, Peter Schultz, Richard Lerner

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.

Original language	English (US)
Pages (from-to)	4008-4011
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	9
DOIs	https://doi.org/10.1073/pnas.90.9.4008
State	Published - May 1 1993
Externally published	Yes

Keywords

Catalysis
Cofactors
Coordination complex
Transgenic mice
Transition metals

ASJC Scopus subject areas

General

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10.1073/pnas.90.9.4008

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abstract = "To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.",

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AU - Sarvetnick, Nora

AU - Gurushanthaiah, Deepak

AU - Han, Nianhe

AU - Prudent, James

AU - Schultz, Peter

AU - Lerner, Richard

PY - 1993/5/1

Y1 - 1993/5/1

N2 - To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.

AB - To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.

KW - Catalysis

KW - Cofactors

KW - Coordination complex

KW - Transgenic mice

KW - Transition metals

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JF - Proceedings of the National Academy of Sciences of the United States of America

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Increasing the chemical potential of the germ-line antibody repertoire

Abstract

Keywords

ASJC Scopus subject areas

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