TY - JOUR
T1 - Indocyanine green loaded hyaluronan-derived nanoparticles for fluorescence-enhanced surgical imaging of pancreatic cancer
AU - Qi, Bowen
AU - Crawford, Ayrianne J.
AU - Wojtynek, Nicholas E.
AU - Holmes, Megan B.
AU - Souchek, Joshua J.
AU - Almeida-Porada, Graca
AU - Ly, Quan P.
AU - Cohen, Samuel M.
AU - Hollingsworth, Michael A.
AU - Mohs, Aaron M.
N1 - Publisher Copyright:
© 2017
PY - 2018/4
Y1 - 2018/4
N2 - Pancreatic ductal adenocarcinoma is highly lethal and surgical resection is the only potential curative treatment for the disease. In this study, hyaluronic acid derived nanoparticles with physico-chemically entrapped indocyanine green, termed NanoICG, were utilized for intraoperative near infrared fluorescence detection of pancreatic cancer. NanoICG was not cytotoxic to healthy pancreatic epithelial cells and did not induce chemotaxis or phagocytosis, it accumulated significantly within the pancreas in an orthotopic pancreatic ductal adenocarcinoma model, and demonstrated contrast-enhancement for pancreatic lesions relative to non-diseased portions of the pancreas. Fluorescence microscopy showed higher fluorescence intensity in pancreatic lesions and splenic metastases due to NanoICG compared to ICG alone. The in vivo safety profile of NanoICG, including, biochemical, hematological, and pathological analysis of NanoICG-treated healthy mice, indicates negligible toxicity. These results suggest that NanoICG is a promising contrast agent for intraoperative detection of pancreatic tumors.
AB - Pancreatic ductal adenocarcinoma is highly lethal and surgical resection is the only potential curative treatment for the disease. In this study, hyaluronic acid derived nanoparticles with physico-chemically entrapped indocyanine green, termed NanoICG, were utilized for intraoperative near infrared fluorescence detection of pancreatic cancer. NanoICG was not cytotoxic to healthy pancreatic epithelial cells and did not induce chemotaxis or phagocytosis, it accumulated significantly within the pancreas in an orthotopic pancreatic ductal adenocarcinoma model, and demonstrated contrast-enhancement for pancreatic lesions relative to non-diseased portions of the pancreas. Fluorescence microscopy showed higher fluorescence intensity in pancreatic lesions and splenic metastases due to NanoICG compared to ICG alone. The in vivo safety profile of NanoICG, including, biochemical, hematological, and pathological analysis of NanoICG-treated healthy mice, indicates negligible toxicity. These results suggest that NanoICG is a promising contrast agent for intraoperative detection of pancreatic tumors.
KW - Fluorescence image-guided surgery
KW - Hyaluronic acid
KW - Indocyanine green
KW - Pancreatic ductal adenocarcinoma
KW - Splenic metastasis
UR - http://www.scopus.com/inward/record.url?scp=85041806849&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85041806849&partnerID=8YFLogxK
U2 - 10.1016/j.nano.2017.12.015
DO - 10.1016/j.nano.2017.12.015
M3 - Article
C2 - 29325740
AN - SCOPUS:85041806849
SN - 1549-9634
VL - 14
SP - 769
EP - 780
JO - Nanomedicine: Nanotechnology, Biology, and Medicine
JF - Nanomedicine: Nanotechnology, Biology, and Medicine
IS - 3
ER -