Induction of apoptosis in murine lymphoma cells by cyclosporin A.

C. Mongini, C. Waldner, E. C. Lopes, M. J. Gravisaco, A. Escalada, M. S. Lockhart, E. Alvarez, S. Hajos

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The aim of this study was to investigate if CsA could induce apoptosis in the murine T-lymphoma cell line LBC, whose growth is inhibited by this immunosuppressive drug. CsA induced programmed cell death in LBC cells with typical features of apoptosis demonstrated by exposure of phosphatidyl serine residues on the cell membrane, the decrease of cell DNA content, chromatin condensation, and nuclear fragmentation. Apoptosis was evident within 12 h after CsA incubation, with a maximal effect at 48 h, in a time and dose-dependent fashion. In addition, the role of apoptosis inhibitors (Bcl-2 and Bcl-x) and the apoptosis inducer (Bax) in CsA induced-apoptosis was evaluated. The expression of Bcl-2 and Bax proteins were high in LBC cells and following CsA treatment the expression of these proteins as well as Bcl-XL decreased. In this work we demonstrated that cell growth inhibition following CsA treatment in LBC was paralleled by the induction of apoptosis thus providing an interesting animal model to identify the mechanism participating in the regulation of apoptotic genes by CsA in T-cell neoplasms and to assess preclinical in vivo trials of T-cell lymphoma-related disorders.

Original languageEnglish (US)
Pages (from-to)431-437
Number of pages7
JournalInternational journal of molecular medicine
Volume7
Issue number4
DOIs
StatePublished - Apr 2001

ASJC Scopus subject areas

  • Genetics

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    Mongini, C., Waldner, C., Lopes, E. C., Gravisaco, M. J., Escalada, A., Lockhart, M. S., Alvarez, E., & Hajos, S. (2001). Induction of apoptosis in murine lymphoma cells by cyclosporin A. International journal of molecular medicine, 7(4), 431-437. https://doi.org/10.3892/ijmm.7.4.431