Induction of hepatic DNA singlestrand breaks in rats by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

Z. Z. Wahba; T. A. Lawson; S. J. Stohs

doi:10.1016/0304-3835(88)90071-7

Induction of hepatic DNA singlestrand breaks in rats by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

Z. Z. Wahba, T. A. Lawson, S. J. Stohs

Eppley Institute for Cancer Research

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Previous studies have demonstrated that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces lipid peroxidation in hepatic and extrahepatic tissues. DNA single strand breaks as well as other forms of DNA damage are believed to occur in conjunction with lipid peroxidation. We have therefore examined the effect of TCDD on hepatic DNA single strand breaks. Ten days after the administration of 100 μg TCDD/kg to female rats, a 7.5-fold increase in the DNA elution constant (single strand breaks) occurred. Similar changes were observed in the content of thiobarbituric acid reactive substances (TBARS) in the nuclei as well as the NADPH-dependent production of TBARS. The accumulation of TBARS appeared to precede the accumulation of DNA single strand breaks. The tumor promoting effects of TCDD may be associated with the enhanced formation of DNA single strand breaks.

Original language	English (US)
Pages (from-to)	281-286
Number of pages	6
Journal	Cancer Letters
Volume	39
Issue number	3
DOIs	https://doi.org/10.1016/0304-3835(88)90071-7
State	Published - Apr 1988

Keywords

2,3,7,8-Tetrachlorodibenzo-p-dioxin
Hepatic DNA single strand breaks
Lipid peroxidation

ASJC Scopus subject areas

Oncology
Cancer Research

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title = "Induction of hepatic DNA singlestrand breaks in rats by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)",

abstract = "Previous studies have demonstrated that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces lipid peroxidation in hepatic and extrahepatic tissues. DNA single strand breaks as well as other forms of DNA damage are believed to occur in conjunction with lipid peroxidation. We have therefore examined the effect of TCDD on hepatic DNA single strand breaks. Ten days after the administration of 100 μg TCDD/kg to female rats, a 7.5-fold increase in the DNA elution constant (single strand breaks) occurred. Similar changes were observed in the content of thiobarbituric acid reactive substances (TBARS) in the nuclei as well as the NADPH-dependent production of TBARS. The accumulation of TBARS appeared to precede the accumulation of DNA single strand breaks. The tumor promoting effects of TCDD may be associated with the enhanced formation of DNA single strand breaks.",

keywords = "2,3,7,8-Tetrachlorodibenzo-p-dioxin, Hepatic DNA single strand breaks, Lipid peroxidation",

author = "Wahba, {Z. Z.} and Lawson, {T. A.} and Stohs, {S. J.}",

note = "Funding Information: These studies were supported in part by a Grant from the March of Dimes Foundation and Grant ES04367 from the National Institute of Environmental Health Sciences.",

year = "1988",

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doi = "10.1016/0304-3835(88)90071-7",

language = "English (US)",

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AU - Wahba, Z. Z.

AU - Lawson, T. A.

AU - Stohs, S. J.

N1 - Funding Information: These studies were supported in part by a Grant from the March of Dimes Foundation and Grant ES04367 from the National Institute of Environmental Health Sciences.

PY - 1988/4

Y1 - 1988/4

N2 - Previous studies have demonstrated that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces lipid peroxidation in hepatic and extrahepatic tissues. DNA single strand breaks as well as other forms of DNA damage are believed to occur in conjunction with lipid peroxidation. We have therefore examined the effect of TCDD on hepatic DNA single strand breaks. Ten days after the administration of 100 μg TCDD/kg to female rats, a 7.5-fold increase in the DNA elution constant (single strand breaks) occurred. Similar changes were observed in the content of thiobarbituric acid reactive substances (TBARS) in the nuclei as well as the NADPH-dependent production of TBARS. The accumulation of TBARS appeared to precede the accumulation of DNA single strand breaks. The tumor promoting effects of TCDD may be associated with the enhanced formation of DNA single strand breaks.

AB - Previous studies have demonstrated that 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) induces lipid peroxidation in hepatic and extrahepatic tissues. DNA single strand breaks as well as other forms of DNA damage are believed to occur in conjunction with lipid peroxidation. We have therefore examined the effect of TCDD on hepatic DNA single strand breaks. Ten days after the administration of 100 μg TCDD/kg to female rats, a 7.5-fold increase in the DNA elution constant (single strand breaks) occurred. Similar changes were observed in the content of thiobarbituric acid reactive substances (TBARS) in the nuclei as well as the NADPH-dependent production of TBARS. The accumulation of TBARS appeared to precede the accumulation of DNA single strand breaks. The tumor promoting effects of TCDD may be associated with the enhanced formation of DNA single strand breaks.

KW - 2,3,7,8-Tetrachlorodibenzo-p-dioxin

KW - Hepatic DNA single strand breaks

KW - Lipid peroxidation

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Induction of hepatic DNA singlestrand breaks in rats by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)

Abstract

Keywords

ASJC Scopus subject areas

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