Induction of mutations in mouse fm3a cells by treatment with riboflavin plus visible light and its possible relation with formation of 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) in dna

Tadayoshi Bessho, Keizo Tano, Susumu Nishimura, Hiroshi Kasai

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19 Scopus citations

Abstract

Photosensitized formation of 8-hydroxyguanine (oh8Gua; 7,8-dihydro-8-oxoguanine, oxo8Gua) in cellular DNA and its repair was examined in mouse FM3A cells. oh8Gua was generated 7-fold above the background level by 10 min irradiation with visible light in the presence of riboflavin (160 μM) and repaired to 60-70% of initial level after 2 h post-incubation. By this treatment, mutation frequency that was measured by counting ouabain-resistant colonies, was increased 7-fold above the background frequency. The accumulation of unrepaired oh8Gua in DNA generated by treatment with riboflavin plus visible light correlates to induction of mutations. Thus, oh8Gua will contribute to the mutation induced by visible light as well as spontaneous mutations.

Original languageEnglish (US)
Pages (from-to)1069-1071
Number of pages3
JournalCarcinogenesis
Volume14
Issue number5
DOIs
StatePublished - May 1993

ASJC Scopus subject areas

  • Cancer Research

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