Induction of Peanut Allergy Through Inhalation of Peanut in Mice

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Peanut (PN) allergy is a common life-threatening disease; however, our knowledge on the immunological mechanisms remains limited. Here, we describe the first mouse model of inhalation-driven peanut allergy. We administered PN flour intranasally to naïve wild-type mice twice a week for 4 weeks, followed by intraperitoneal challenge with PN extract. Exposure of mice to PN flour sensitized them without addition of adjuvants, and mice developed PN-specific IgE, IgG1, and IgG2a. After challenge, mice displayed lower body temperature and other clinical signs of anaphylaxis. This inhalation model is an ideal system to allow for future examination of immunological mechanisms critical for the development of PN allergy.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages19-35
Number of pages17
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2223
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Allergy
  • Anaphylaxis
  • Food allergy
  • IgE
  • Inhalation
  • Peanut

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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