TY - JOUR
T1 - Infection of baboon microglia with SIV-HIV recombinant viruses
T2 - Role of CD4 and chemokine receptors
AU - Kanmogne, Georgette D.
AU - Kennedy, Ronald C.
AU - Grammas, Paula
PY - 2002
Y1 - 2002
N2 - Microglia constitute the primary cell type infected with HIV in the brain and play a major role in viral persistence in the CNS and in the development of AIDS dementia. Lack of a suitable animal model and limitations in the availability of human tissues hinder most HIV/AIDS studies investigating the neuropathogenesis of AIDS dementia. The aims of this study were to determine whether baboon microglia can be productively infected with SIV-HIV (SHIV) recombinant viruses in vitro and whether they express HIV-1 receptors and coreceptors. Our results show the presence of mRNA for CD4, CCR5, and CXCR4 chemokine receptors on baboon microglial cells. Microglia lacked mRNA for the CCR3 chemokine receptor. We also show productive infection of baboon microglial cells by two SHIV isolates, SHIV-KU and SHIV-89.6P, and blockade of the infection with soluble CD4 protein, CCR5, and CXCR4 monoclonal antibodies. This study demonstrating the feasibility of infecting baboon microglia with SHIV isolates is an important first step in using the baboon as an alternative nonhuman primate model to study HIV neuropathogenesis.
AB - Microglia constitute the primary cell type infected with HIV in the brain and play a major role in viral persistence in the CNS and in the development of AIDS dementia. Lack of a suitable animal model and limitations in the availability of human tissues hinder most HIV/AIDS studies investigating the neuropathogenesis of AIDS dementia. The aims of this study were to determine whether baboon microglia can be productively infected with SIV-HIV (SHIV) recombinant viruses in vitro and whether they express HIV-1 receptors and coreceptors. Our results show the presence of mRNA for CD4, CCR5, and CXCR4 chemokine receptors on baboon microglial cells. Microglia lacked mRNA for the CCR3 chemokine receptor. We also show productive infection of baboon microglial cells by two SHIV isolates, SHIV-KU and SHIV-89.6P, and blockade of the infection with soluble CD4 protein, CCR5, and CXCR4 monoclonal antibodies. This study demonstrating the feasibility of infecting baboon microglia with SHIV isolates is an important first step in using the baboon as an alternative nonhuman primate model to study HIV neuropathogenesis.
UR - http://www.scopus.com/inward/record.url?scp=0036264381&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036264381&partnerID=8YFLogxK
U2 - 10.1089/088922202753747905
DO - 10.1089/088922202753747905
M3 - Article
C2 - 12036485
AN - SCOPUS:0036264381
SN - 0889-2229
VL - 18
SP - 557
EP - 565
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 8
ER -