Infectious molecular clone of a recently transmitted pediatric human immunodeficiency virus clade C isolate from Africa: Evidence of intraclade recombination

Ricky D. Grisson, Agnès Laurence Chenine, Lan Yu Yeh, Jun He, Charles Wood, Ganapati J. Bhat, Weidong Xu, Chipepo Kankasa, Ruth M. Ruprecht

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Although human immunodeficiency virus type 1 (HFV-1) clade C continues to dominate the pandemic, only two infectious clade C proviral DNA clones have been described (N. Mochizuki, N. Otsuka, K. Matsuo, T. Shiino, A. Kojima, T. Kurata, K. Sakai, N. Yamamoto, S. Isomura, T. N. Dhole, Y. Takebe, M. Matsuda, and M. Tatsumi, AIDS Res. Hum. Retrovir. 15:1321-1324, 1999; T. Ndung'u, B. Renjifo, and M. Essex, J. Virol. 75:4964-4972, 2001). We have generated an infectious molecular clone of a pediatric clade C strain, HIV1084i, which was isolated from a Zambian infant infected either intrapartum or through breastfeeding. HIV1084i is an R5, non-syncytium-inducing isolate that bears all known clade C signatures; gag, pol, and env consistently mapped within clade C. Interestingly, gag resembled Asian isolates, whereas pol and env resembled African isolates, indicating that HIV1084i probably arose from an intraclade recombination. As a recently transmitted clade C strain, HIV1084i will be a useful vaccine development tool.

Original languageEnglish (US)
Pages (from-to)14066-14069
Number of pages4
JournalJournal of virology
Volume78
Issue number24
DOIs
StatePublished - Dec 2004

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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