TY - JOUR
T1 - Infectious molecular clone of a recently transmitted pediatric human immunodeficiency virus clade C isolate from Africa
T2 - Evidence of intraclade recombination
AU - Grisson, Ricky D.
AU - Chenine, Agnès Laurence
AU - Yeh, Lan Yu
AU - He, Jun
AU - Wood, Charles
AU - Bhat, Ganapati J.
AU - Xu, Weidong
AU - Kankasa, Chipepo
AU - Ruprecht, Ruth M.
PY - 2004/12
Y1 - 2004/12
N2 - Although human immunodeficiency virus type 1 (HFV-1) clade C continues to dominate the pandemic, only two infectious clade C proviral DNA clones have been described (N. Mochizuki, N. Otsuka, K. Matsuo, T. Shiino, A. Kojima, T. Kurata, K. Sakai, N. Yamamoto, S. Isomura, T. N. Dhole, Y. Takebe, M. Matsuda, and M. Tatsumi, AIDS Res. Hum. Retrovir. 15:1321-1324, 1999; T. Ndung'u, B. Renjifo, and M. Essex, J. Virol. 75:4964-4972, 2001). We have generated an infectious molecular clone of a pediatric clade C strain, HIV1084i, which was isolated from a Zambian infant infected either intrapartum or through breastfeeding. HIV1084i is an R5, non-syncytium-inducing isolate that bears all known clade C signatures; gag, pol, and env consistently mapped within clade C. Interestingly, gag resembled Asian isolates, whereas pol and env resembled African isolates, indicating that HIV1084i probably arose from an intraclade recombination. As a recently transmitted clade C strain, HIV1084i will be a useful vaccine development tool.
AB - Although human immunodeficiency virus type 1 (HFV-1) clade C continues to dominate the pandemic, only two infectious clade C proviral DNA clones have been described (N. Mochizuki, N. Otsuka, K. Matsuo, T. Shiino, A. Kojima, T. Kurata, K. Sakai, N. Yamamoto, S. Isomura, T. N. Dhole, Y. Takebe, M. Matsuda, and M. Tatsumi, AIDS Res. Hum. Retrovir. 15:1321-1324, 1999; T. Ndung'u, B. Renjifo, and M. Essex, J. Virol. 75:4964-4972, 2001). We have generated an infectious molecular clone of a pediatric clade C strain, HIV1084i, which was isolated from a Zambian infant infected either intrapartum or through breastfeeding. HIV1084i is an R5, non-syncytium-inducing isolate that bears all known clade C signatures; gag, pol, and env consistently mapped within clade C. Interestingly, gag resembled Asian isolates, whereas pol and env resembled African isolates, indicating that HIV1084i probably arose from an intraclade recombination. As a recently transmitted clade C strain, HIV1084i will be a useful vaccine development tool.
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U2 - 10.1128/JVI.78.24.14066-14069.2004
DO - 10.1128/JVI.78.24.14066-14069.2004
M3 - Article
C2 - 15564517
AN - SCOPUS:10044223604
SN - 0022-538X
VL - 78
SP - 14066
EP - 14069
JO - Journal of Virology
JF - Journal of Virology
IS - 24
ER -