Infectivity of cryopreserved Giardia cysts for Mongolian gerbils (Meriones unguiculatus)

J. W. Dickerson, G. S. Visvesvara, E. M. Walker, D. E. Feely

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Although Giardia species trophozoites have been cryopreserved successfully, no report on the successful cryopreservation of cysts could be found. Using infectivity to Mongolian gerbils (Meriones unguiculatus) as a measure of cyst viability, we tested the viability of 4 strains of Giardia cysts that had been cryopreserved for 1-67 wk. Cysts were frozen in either Keister's medium or physiological saline, both containing 5% or 7.5% dimethylsulfoxide as the cryoprotectant. Viability of cryopreserved cysts was dependent upon the number of cysts inoculated, the length of time cysts were held at 4 C before cryopreservation, and the cryopreserving medium. Infection was established in gerbils by inoculating them with cysts that had been cryopreserved for up to 67 wk, cysts that had been held for less than 30 days before cryopreservation, and cysts frozen in Keister's medium. Saline appears to be unsuitable as a freezing medium for the cryopreservation of Giardia cysts.

Original languageEnglish (US)
Pages (from-to)688-691
Number of pages4
JournalJournal of Parasitology
Volume77
Issue number5
DOIs
StatePublished - Dec 16 1991

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ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

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