Influence of age, irradiation and humanization on NSG mouse phenotypes

Jaclyn S. Knibbe-Hollinger, Natasha R. Fields, Tammy R. Chaudoin, Adrian A. Epstein, Edward Makarov, Sidra P. Akhter, Santhi Gorantla, Stephen J. Bonasera, Howard E. Gendelman, Larisa Y. Poluektova

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Humanized mice are frequently utilized in bench to bedside therapeutic tests to combat human infectious, cancerous and degenerative diseases. For the fields of hematology-oncology, regenerative medicine, and infectious diseases, the immune deficient mice have been used commonly in basic research efforts. Obstacles in true translational efforts abound, as the relationship between mouse and human cells in disease pathogenesis and therapeutic studies requires lengthy investigations. The interplay between human immunity and mouse biology proves ever more complicated when aging, irradiation, and human immune reconstitution are considered. All can affect a range of biochemical and behavioral functions. To such ends, we show age- and irradiation-dependent influences for the development of macrocytic hyper chromic anemia, myelodysplasia, blood protein reductions and body composition changes. Humanization contributes to hematologic abnormalities. Home cage behavior revealed day and dark cycle locomotion also influenced by human cell reconstitutions. Significant age-related day-to-day variability in movement, feeding and drinking behaviors were observed. We posit that this data serves to enable researchers to better design translational studies in this rapidly emerging field of mouse humanization.

Original languageEnglish (US)
Pages (from-to)1243-1252
Number of pages10
JournalBiology Open
Volume4
Issue number10
DOIs
StatePublished - 2015

Keywords

  • Behavior
  • Body mass composition
  • Chemistry
  • Circadian rhythm
  • Hematology
  • Locomotion
  • Mouse home cage monitoring
  • Nsg mice
  • Time

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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  • Cite this

    Knibbe-Hollinger, J. S., Fields, N. R., Chaudoin, T. R., Epstein, A. A., Makarov, E., Akhter, S. P., Gorantla, S., Bonasera, S. J., Gendelman, H. E., & Poluektova, L. Y. (2015). Influence of age, irradiation and humanization on NSG mouse phenotypes. Biology Open, 4(10), 1243-1252. https://doi.org/10.1242/bio.013201