TY - JOUR
T1 - Influence of prion strain on prion protein adsorption to soil in a competitive matrix
AU - Saunders, Samuel E.
AU - Bartz, Jason C.
AU - Bartelt-Hunt, Shannon L.
PY - 2009/7/15
Y1 - 2009/7/15
N2 - It is likely that the soil environment serves as a stable reservoir of infectious chronic wasting disease (CWD) and scrapie prions,aswellasapotential reservoir of bovine spongiform encephalopathy (BSE, or "mad cow" disease). Prion adsorption to soil may play an important role in prion mobility, proteolysis, and infectivity. Differences in PrP environmental fate are possible due to the strain- and species-dependent structure of PrPSc. Kinetic and isothermal studies of PrP adsorption to sand and two whole soils were conducted using HY and DY TME-infected hamster, uninfected hamster, and CWD-infected elk brain homogenates as competitive PrP sources. The role of the N-terminus in PrP adsorption was also investigated. We report strain and species differences in PrP adsorption to soil over time and as a function of aqueous concentration, indicating that the fate of prions in the environment may vary with the prion strain and species infected. Our data also provide evidence that the N-terminal region of PrP enhances adsorption to clay but may hinder adsorption to sand. PrP adsorption was maximal at an intermediate aqueous concentration, most likely due to the competitive brain homogenate matrix in which it enters the soil environment.
AB - It is likely that the soil environment serves as a stable reservoir of infectious chronic wasting disease (CWD) and scrapie prions,aswellasapotential reservoir of bovine spongiform encephalopathy (BSE, or "mad cow" disease). Prion adsorption to soil may play an important role in prion mobility, proteolysis, and infectivity. Differences in PrP environmental fate are possible due to the strain- and species-dependent structure of PrPSc. Kinetic and isothermal studies of PrP adsorption to sand and two whole soils were conducted using HY and DY TME-infected hamster, uninfected hamster, and CWD-infected elk brain homogenates as competitive PrP sources. The role of the N-terminus in PrP adsorption was also investigated. We report strain and species differences in PrP adsorption to soil over time and as a function of aqueous concentration, indicating that the fate of prions in the environment may vary with the prion strain and species infected. Our data also provide evidence that the N-terminal region of PrP enhances adsorption to clay but may hinder adsorption to sand. PrP adsorption was maximal at an intermediate aqueous concentration, most likely due to the competitive brain homogenate matrix in which it enters the soil environment.
UR - http://www.scopus.com/inward/record.url?scp=67650456832&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67650456832&partnerID=8YFLogxK
U2 - 10.1021/es900502f
DO - 10.1021/es900502f
M3 - Article
C2 - 19708348
AN - SCOPUS:67650456832
SN - 0013-936X
VL - 43
SP - 5242
EP - 5248
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 14
ER -