Infrared and visible ellipsometric studies of cholera toxin in ELISA structures

Daniel W. Thompson; Galen Pfeiffer; Emil Berberov; Leon Castro; John A. Woollam

doi:10.1117/12.479262

Infrared and visible ellipsometric studies of cholera toxin in ELISA structures

Daniel W. Thompson, Galen Pfeiffer, Emil Berberov, Leon Castro, John A. Woollam

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

Ellipsometry is well known for its extreme sensitivity to the presence and properties of ultra-thin films. In the infrared, resonance response to chemical bonds allows chemical identification in monolayer-thick biological films. In this paper we show results of attachment repeatability for successive layers of monosialoganglioside, cholera toxin, and related antibodies using in situ visible spectroscopic ellipsometry. Several factors contributing to difficulty in obtaining reproducible results are discussed. Specifically, these include freshness of reagents; surface type, cleaning, and preparation; temperature; birefringence of liquid cell windows; and cell design. Sensitivity and signal noise considerations for infrared spectra of molecular monolayers are discussed.

Original language	English (US)
Pages (from-to)	138-146
Number of pages	9
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	4965
DOIs	https://doi.org/10.1117/12.479262
State	Published - 2003
Externally published	Yes
Event	PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Diagnostics and Sensing in Biomedicine III - San Jose, CA, United States Duration: Jan 28 2003 → Jan 29 2003

Keywords

Biological films
Cholera toxin
ELISA
Ellipsometry

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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10.1117/12.479262

Cite this

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title = "Infrared and visible ellipsometric studies of cholera toxin in ELISA structures",

abstract = "Ellipsometry is well known for its extreme sensitivity to the presence and properties of ultra-thin films. In the infrared, resonance response to chemical bonds allows chemical identification in monolayer-thick biological films. In this paper we show results of attachment repeatability for successive layers of monosialoganglioside, cholera toxin, and related antibodies using in situ visible spectroscopic ellipsometry. Several factors contributing to difficulty in obtaining reproducible results are discussed. Specifically, these include freshness of reagents; surface type, cleaning, and preparation; temperature; birefringence of liquid cell windows; and cell design. Sensitivity and signal noise considerations for infrared spectra of molecular monolayers are discussed.",

keywords = "Biological films, Cholera toxin, ELISA, Ellipsometry",

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language = "English (US)",

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journal = "Proceedings of SPIE - The International Society for Optical Engineering",

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T1 - Infrared and visible ellipsometric studies of cholera toxin in ELISA structures

AU - Thompson, Daniel W.

AU - Pfeiffer, Galen

AU - Berberov, Emil

AU - Castro, Leon

AU - Woollam, John A.

PY - 2003

Y1 - 2003

N2 - Ellipsometry is well known for its extreme sensitivity to the presence and properties of ultra-thin films. In the infrared, resonance response to chemical bonds allows chemical identification in monolayer-thick biological films. In this paper we show results of attachment repeatability for successive layers of monosialoganglioside, cholera toxin, and related antibodies using in situ visible spectroscopic ellipsometry. Several factors contributing to difficulty in obtaining reproducible results are discussed. Specifically, these include freshness of reagents; surface type, cleaning, and preparation; temperature; birefringence of liquid cell windows; and cell design. Sensitivity and signal noise considerations for infrared spectra of molecular monolayers are discussed.

AB - Ellipsometry is well known for its extreme sensitivity to the presence and properties of ultra-thin films. In the infrared, resonance response to chemical bonds allows chemical identification in monolayer-thick biological films. In this paper we show results of attachment repeatability for successive layers of monosialoganglioside, cholera toxin, and related antibodies using in situ visible spectroscopic ellipsometry. Several factors contributing to difficulty in obtaining reproducible results are discussed. Specifically, these include freshness of reagents; surface type, cleaning, and preparation; temperature; birefringence of liquid cell windows; and cell design. Sensitivity and signal noise considerations for infrared spectra of molecular monolayers are discussed.

KW - Biological films

KW - Cholera toxin

KW - ELISA

KW - Ellipsometry

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DO - 10.1117/12.479262

M3 - Conference article

AN - SCOPUS:0345714693

SN - 0277-786X

VL - 4965

SP - 138

EP - 146

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

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Y2 - 28 January 2003 through 29 January 2003

ER -

Infrared and visible ellipsometric studies of cholera toxin in ELISA structures

Abstract

Keywords

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