Ingress of blood-borne macrophages across the blood-brain barrier in murine HIV-1 encephalitis

Yutong Liu; Mariano G. Uberti; Huanyu Dou; Rebecca Banerjee; Cassi B. Grotepas; David K. Stone; Barrett E. Rabinow; Howard E. Gendelman; Michael D. Boska

doi:10.1016/j.jneuroim.2008.06.009

Ingress of blood-borne macrophages across the blood-brain barrier in murine HIV-1 encephalitis

Yutong Liu, Mariano G. Uberti, Huanyu Dou, Rebecca Banerjee, Cassi B. Grotepas, David K. Stone, Barrett E. Rabinow, Howard E. Gendelman, Michael D. Boska

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Blood-borne macrophage ingress into brain in HIV-1 associated neurocognitive disorders governs the tempo of disease. We used superparamagnetic iron-oxide particles loaded into murine bone marrow-derived macrophages (BMM) injected intravenously into HIV-1 encephalitis mice to quantitatively assess BMM entry into diseased brain regions. Magnetic resonance imaging tests were validated by histological coregistration and enhanced image processing. The demonstration of robust BMM migration into areas of focal encephalitis provide 'proof of concept' for the use of MRI to monitor macrophage ingress into brain.

Original language	English (US)
Pages (from-to)	41-52
Number of pages	12
Journal	Journal of Neuroimmunology
Volume	200
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jneuroim.2008.06.009
State	Published - Aug 30 2008

Keywords

Blood-brain barrier
HIV-1 encephalitis
Image coregistration
Monocyte-derived macrophages

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2008.06.009

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title = "Ingress of blood-borne macrophages across the blood-brain barrier in murine HIV-1 encephalitis",

abstract = "Blood-borne macrophage ingress into brain in HIV-1 associated neurocognitive disorders governs the tempo of disease. We used superparamagnetic iron-oxide particles loaded into murine bone marrow-derived macrophages (BMM) injected intravenously into HIV-1 encephalitis mice to quantitatively assess BMM entry into diseased brain regions. Magnetic resonance imaging tests were validated by histological coregistration and enhanced image processing. The demonstration of robust BMM migration into areas of focal encephalitis provide 'proof of concept' for the use of MRI to monitor macrophage ingress into brain.",

keywords = "Blood-brain barrier, HIV-1 encephalitis, Image coregistration, Monocyte-derived macrophages",

author = "Yutong Liu and Uberti, {Mariano G.} and Huanyu Dou and Rebecca Banerjee and Grotepas, {Cassi B.} and Stone, {David K.} and Rabinow, {Barrett E.} and Gendelman, {Howard E.} and Boska, {Michael D.}",

note = "Funding Information: The work was supported by the Nebraska Research Initiative (to M.D.B.), the Francine and Louis Blumkin Foundation, the Community Neuroscience Pride of Nebraska Initiative, the Carol Swartz, M.D. Neuroscience Laboratory, and the Alan Baer Charitable Trust and NIH grants R01 NS034239, P01 NS31492, 1054 NS43011 (to H.E.G.) and P01MH64570, R37 NS36126, P01 NS 43985, and P20 RR15635 (to H.E.G. and M.D.B.). ",

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doi = "10.1016/j.jneuroim.2008.06.009",

language = "English (US)",

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AU - Liu, Yutong

AU - Uberti, Mariano G.

AU - Dou, Huanyu

AU - Banerjee, Rebecca

AU - Grotepas, Cassi B.

AU - Stone, David K.

AU - Rabinow, Barrett E.

AU - Gendelman, Howard E.

AU - Boska, Michael D.

N1 - Funding Information: The work was supported by the Nebraska Research Initiative (to M.D.B.), the Francine and Louis Blumkin Foundation, the Community Neuroscience Pride of Nebraska Initiative, the Carol Swartz, M.D. Neuroscience Laboratory, and the Alan Baer Charitable Trust and NIH grants R01 NS034239, P01 NS31492, 1054 NS43011 (to H.E.G.) and P01MH64570, R37 NS36126, P01 NS 43985, and P20 RR15635 (to H.E.G. and M.D.B.).

PY - 2008/8/30

Y1 - 2008/8/30

N2 - Blood-borne macrophage ingress into brain in HIV-1 associated neurocognitive disorders governs the tempo of disease. We used superparamagnetic iron-oxide particles loaded into murine bone marrow-derived macrophages (BMM) injected intravenously into HIV-1 encephalitis mice to quantitatively assess BMM entry into diseased brain regions. Magnetic resonance imaging tests were validated by histological coregistration and enhanced image processing. The demonstration of robust BMM migration into areas of focal encephalitis provide 'proof of concept' for the use of MRI to monitor macrophage ingress into brain.

AB - Blood-borne macrophage ingress into brain in HIV-1 associated neurocognitive disorders governs the tempo of disease. We used superparamagnetic iron-oxide particles loaded into murine bone marrow-derived macrophages (BMM) injected intravenously into HIV-1 encephalitis mice to quantitatively assess BMM entry into diseased brain regions. Magnetic resonance imaging tests were validated by histological coregistration and enhanced image processing. The demonstration of robust BMM migration into areas of focal encephalitis provide 'proof of concept' for the use of MRI to monitor macrophage ingress into brain.

KW - Blood-brain barrier

KW - HIV-1 encephalitis

KW - Image coregistration

KW - Monocyte-derived macrophages

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Ingress of blood-borne macrophages across the blood-brain barrier in murine HIV-1 encephalitis

Abstract

Keywords

ASJC Scopus subject areas

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