Inhibition of carotid artery neointimal formation with intravenous microbubbles

Thomas R. Porter, William L. Hiser, David Kricsfeld, Ubeydullah Deligonul, Feng Xie, Patrick Iversen, Stanley Radio

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Because therapeutic gene products such as synthetic antisense oligodeoxynucleotides (ODN) bind to albumin-coated microbubbles, we sought to determine whether IV perfluorocarbon-exposed sonicated dextrose albumin (PESDA) microbubbles could target their delivery to the carotid artery following balloon injury. In 5 pigs, the concentration of ODN taken up within the carotid vascular wall was found to be significantly increased when the IV antisense (ODN) was administered bound to PESDA (ODN-PESDA), and while transcutaneous low-frequency (20 kHz) ultrasound was applied over the carotid artery. Based on these results, a chronic model was then developed, in which 21 pigs received either IV ODN-PESDA, ODN alone, or control, following carotid balloon injury. At 30 days following balloon injury, percent area stenosis was only 8 ± 2% in the ODN-PESDA groups compared to 19 ± 8% and 28 ± 3% in the other groups (p < 0.01). IV PESDA may be a method of noninvasively targeting the delivery of therapeutic genes. (E-mail: trporter@unmc.edu)

Original languageEnglish (US)
Pages (from-to)259-265
Number of pages7
JournalUltrasound in Medicine and Biology
Volume27
Issue number2
DOIs
StatePublished - 2001

Keywords

  • Antisense oligonucleotides
  • Drug delivery
  • Microbubbles
  • Ultrasound

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics

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