Inhibition of herpes simplex virus 1 reproduction with hydrophobized antisense oligonucleotides

Sergey V. Vinogradov, Yulia Suzdaltseva, Valery Yu Alakhov, Alexander V. Kabanov

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Antisense oligonucleotides were modified by their ends with hydrophobic substituents which permitted enhancing their activity. The effect of hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus type 1 in Vero cells was studied. Two types of oligonucleotides were used: a 12-mer complementary to the splicing site 983-994 of early mRNA-5 of the virus and 19-mer complementary to the site of mRNA that encodes the virus DNA-polymerase. These oligonucleotides were modified by 5’-ends with n-undecyl or cholesteryl moieties and by 3’-ends with acridine or 1,7-heptanediol. In comparison with the unnmodified oligonucleotides the hydrophobized ones were significantly more active and inhibited HSV-1 infection at micromolar concentrations.

Original languageEnglish (US)
Pages (from-to)959-966
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume203
Issue number2
DOIs
StatePublished - Sep 15 1994

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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