TY - JOUR
T1 - Inhibition of herpes simplex virus 1 reproduction with hydrophobized antisense oligonucleotides
AU - Vinogradov, Sergey V.
AU - Suzdaltseva, Yulia
AU - Alakhov, Valery Yu
AU - Kabanov, Alexander V.
PY - 1994/9/15
Y1 - 1994/9/15
N2 - Antisense oligonucleotides were modified by their ends with hydrophobic substituents which permitted enhancing their activity. The effect of hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus type 1 in Vero cells was studied. Two types of oligonucleotides were used: a 12-mer complementary to the splicing site 983-994 of early mRNA-5 of the virus and 19-mer complementary to the site of mRNA that encodes the virus DNA-polymerase. These oligonucleotides were modified by 5’-ends with n-undecyl or cholesteryl moieties and by 3’-ends with acridine or 1,7-heptanediol. In comparison with the unnmodified oligonucleotides the hydrophobized ones were significantly more active and inhibited HSV-1 infection at micromolar concentrations.
AB - Antisense oligonucleotides were modified by their ends with hydrophobic substituents which permitted enhancing their activity. The effect of hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus type 1 in Vero cells was studied. Two types of oligonucleotides were used: a 12-mer complementary to the splicing site 983-994 of early mRNA-5 of the virus and 19-mer complementary to the site of mRNA that encodes the virus DNA-polymerase. These oligonucleotides were modified by 5’-ends with n-undecyl or cholesteryl moieties and by 3’-ends with acridine or 1,7-heptanediol. In comparison with the unnmodified oligonucleotides the hydrophobized ones were significantly more active and inhibited HSV-1 infection at micromolar concentrations.
UR - http://www.scopus.com/inward/record.url?scp=0027935039&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027935039&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1994.2275
DO - 10.1006/bbrc.1994.2275
M3 - Article
C2 - 8093080
AN - SCOPUS:0027935039
SN - 0006-291X
VL - 203
SP - 959
EP - 966
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -