Inhibition of herpes simplex virus 1 reproduction with hydrophobized antisense oligonucleotides

Sergey V. Vinogradov; Yulia Suzdaltseva; Valery Yu Alakhov; Alexander V. Kabanov

doi:10.1006/bbrc.1994.2275

Inhibition of herpes simplex virus 1 reproduction with hydrophobized antisense oligonucleotides

Sergey V. Vinogradov, Yulia Suzdaltseva, Valery Yu Alakhov, Alexander V. Kabanov

Pharmaceutical Science

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Antisense oligonucleotides were modified by their ends with hydrophobic substituents which permitted enhancing their activity. The effect of hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus type 1 in Vero cells was studied. Two types of oligonucleotides were used: a 12-mer complementary to the splicing site 983-994 of early mRNA-5 of the virus and 19-mer complementary to the site of mRNA that encodes the virus DNA-polymerase. These oligonucleotides were modified by 5’-ends with n-undecyl or cholesteryl moieties and by 3’-ends with acridine or 1,7-heptanediol. In comparison with the unnmodified oligonucleotides the hydrophobized ones were significantly more active and inhibited HSV-1 infection at micromolar concentrations.

Original language	English (US)
Pages (from-to)	959-966
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	203
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1994.2275
State	Published - Sep 15 1994

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "Antisense oligonucleotides were modified by their ends with hydrophobic substituents which permitted enhancing their activity. The effect of hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus type 1 in Vero cells was studied. Two types of oligonucleotides were used: a 12-mer complementary to the splicing site 983-994 of early mRNA-5 of the virus and 19-mer complementary to the site of mRNA that encodes the virus DNA-polymerase. These oligonucleotides were modified by 5{\textquoteright}-ends with n-undecyl or cholesteryl moieties and by 3{\textquoteright}-ends with acridine or 1,7-heptanediol. In comparison with the unnmodified oligonucleotides the hydrophobized ones were significantly more active and inhibited HSV-1 infection at micromolar concentrations.",

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T1 - Inhibition of herpes simplex virus 1 reproduction with hydrophobized antisense oligonucleotides

AU - Vinogradov, Sergey V.

AU - Suzdaltseva, Yulia

AU - Alakhov, Valery Yu

AU - Kabanov, Alexander V.

PY - 1994/9/15

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AB - Antisense oligonucleotides were modified by their ends with hydrophobic substituents which permitted enhancing their activity. The effect of hydrophobized oligonucleotides on reproduction of Herpes Simplex Virus type 1 in Vero cells was studied. Two types of oligonucleotides were used: a 12-mer complementary to the splicing site 983-994 of early mRNA-5 of the virus and 19-mer complementary to the site of mRNA that encodes the virus DNA-polymerase. These oligonucleotides were modified by 5’-ends with n-undecyl or cholesteryl moieties and by 3’-ends with acridine or 1,7-heptanediol. In comparison with the unnmodified oligonucleotides the hydrophobized ones were significantly more active and inhibited HSV-1 infection at micromolar concentrations.

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Inhibition of herpes simplex virus 1 reproduction with hydrophobized antisense oligonucleotides

Abstract

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